Team:Caltech/Notebook/Proteorhodopsin

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Daisy - ordered primers for Polymerase Cycling Assembly (PCA) reaction to create a proteorhodopsin optimized for E. coli
Daisy - ordered primers for Polymerase Cycling Assembly (PCA) reaction to create a proteorhodopsin optimized for E. coli
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<div style="width:image width px; font-size:80%;"><img src="https://static.igem.org/mediawiki/2012/d/d4/Daisy.jpg" alt="alternate text" width="width" height="height" style="padding-bottom:0.5em;" /><br>This is my caption</div>
 
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<a a style="float:right;" href="https://2012.igem.org/Team:Caltech/Notebook/Proteorhodopsin#Calendar">Back to Proteorhodopsin Notebook Calendar</a>
<a a style="float:right;" href="https://2012.igem.org/Team:Caltech/Notebook/Proteorhodopsin#Calendar">Back to Proteorhodopsin Notebook Calendar</a>
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<font size="+2"><a name="6_26_12">6/26/12</a></font>
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Chenxi - PCR pKD4 kan for nuo and ndh; ran gel
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Revision as of 22:42, 13 July 2012




Proteorhodopsin Notebook Calendar

June 2012
Sun Mon Tue Wed Thu Fri Sat
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3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
July 2012
Sun Mon Tue Wed Thu Fri Sat
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31
August 2012
Sun Mon Tue Wed Thu Fri Sat
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5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31


Proteorhodopsin Notebook


6/22/12

Julia - transformed K572005 (proteorhodopsin gene)
Daisy - ordered primers for Polymerase Cycling Assembly (PCA) reaction to create a proteorhodopsin optimized for E. coli
Back to Proteorhodopsin Notebook Calendar
6/25/12

Chenxi - Transformed pKD46 into wild type MG1655 E.coli strain using electroporation; designed primers to PCR pKD4 Kan resistance gene for lambda red knockout of nuo and ndh genes (NADH dehydrogenases)
Julia - sequenced K572005
Back to Proteorhodopsin Notebook Calendar
6/26/12

Chenxi - PCR pKD4 kan for nuo and ndh; ran gel
Back to Proteorhodopsin Notebook Calendar
6/27/12

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6/28/12

Back to Proteorhodopsin Notebook Calendar
6/29/12

Back to Proteorhodopsin Notebook Calendar
6/30/12

Back to Proteorhodopsin Notebook Calendar
7/1/12

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7/2/12

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7/3/12

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