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Team:Colombia/Notebook/Protocols
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! Amount (μL) | ! Amount (μL) | ||
|- | |- | ||
| - | |H~2~O | + | |H ~2~ O |
|10 | |10 | ||
|- | |- | ||
| - | | | + | |Buffer |
|4 | |4 | ||
|- | |- | ||
| - | | | + | |MgSO_4 |
|1 | |1 | ||
|- | |- | ||
Revision as of 21:43, 13 July 2012
Contents |
Bacterial DNA extraction protocol
Miniprep
Electroporation
Electro-competent cells of E. coli DH5α are electroporated at 1.25 mV, immediately we add SOC medium to the bacteria and we incubated on a shaker at 37°C for one hour, then, we culture cells in a solid medium containing the antibiotic of selection.
PCR - Pfu DNA polimerase
| Reactives | Amount (μL) |
|---|---|
| H ~2~ O | 10 |
| Buffer | 4 |
| MgSO_4 | 1 |
