Team:Colombia/Notebook/Protocols

From 2012.igem.org

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=='''PCR - Pfu DNA polimerase'''==
=='''PCR - Pfu DNA polimerase'''==
 +
 +
{|
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! align="left"| Reactives
 +
! Amount (μL)
 +
|-
 +
|H~2~O
 +
|10
 +
|-
 +
|Bread
 +
|4
 +
|-
 +
|Butter
 +
|1
 +
|-
 +
|}
=='''PCR - Invitrogen (R) Taq polimerase'''==
=='''PCR - Invitrogen (R) Taq polimerase'''==

Revision as of 21:43, 13 July 2012

Contents

Bacterial DNA extraction protocol

Miniprep

Electroporation

Electro-competent cells of E. coli DH5α are electroporated at 1.25 mV, immediately we add SOC medium to the bacteria and we incubated on a shaker at 37°C for one hour, then, we culture cells in a solid medium containing the antibiotic of selection.

PCR - Pfu DNA polimerase

Reactives Amount (μL)
H~2~O 10
Bread 4
Butter 1

PCR - Invitrogen (R) Taq polimerase

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