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Team:Cambridge/Protocols
From 2012.igem.org
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* [[Team:Cambridge/Protocols/RestrictionDigest| Restriction Enzyme Digest]] A method for creating a restriction map of a plasmid. | * [[Team:Cambridge/Protocols/RestrictionDigest| Restriction Enzyme Digest]] A method for creating a restriction map of a plasmid. | ||
* [[Team:Cambridge/Protocols/SDSPAGE| Protein analysis by SDS PAGE]] A method used to separate polypeptides of different lengths. | * [[Team:Cambridge/Protocols/SDSPAGE| Protein analysis by SDS PAGE]] A method used to separate polypeptides of different lengths. | ||
| + | * [[Team:Cambridge/Protocols/Plates| Preparation of LB Agar Plates]] A method used to prepare agar plate to culture common bacteria. | ||
'''N.B.''' Information in purple is subject to change through optimisation over the course of our project. | '''N.B.''' Information in purple is subject to change through optimisation over the course of our project. | ||
Revision as of 16:02, 13 July 2012
- PCR using Phusion DNA polymerase A method for amplifying a section of DNA.
- Colony PCR PCR with cells as a template. Useful for checking the length of an insert in an introduced plasmid.
- Gel Electrophoresis A technique for separating DNA strands of different lengths.
- Gel Extraction of DNA A technique used to isolate a desired fragment of intact DNA from an agarose gel following agarose gel electrophoresis.
- Gibson Assembly A technique for ligating multiple DNA fragments in one step, compatible with standard assembly.
- Transformation of Bacillus subtilis A technique used to introduce foreign DNA into competent Bacillus cells.
- MiniPrep A method used to extract DNA from bacterial cells.
- Restriction Enzyme Digest A method for creating a restriction map of a plasmid.
- Protein analysis by SDS PAGE A method used to separate polypeptides of different lengths.
- Preparation of LB Agar Plates A method used to prepare agar plate to culture common bacteria.
N.B. Information in purple is subject to change through optimisation over the course of our project.
