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Team:Cambridge/Week 3/Lab book
From 2012.igem.org
(Difference between revisions)
| Line 25: | Line 25: | ||
---- | ---- | ||
| - | ''Bacillus salts'' *10 made up: | + | '''Bacillus salts''' *10 made up: |
*(NH4)2SO4: 20g | *(NH4)2SO4: 20g | ||
| Line 33: | Line 33: | ||
* Tri-sodium citrate 10g | * Tri-sodium citrate 10g | ||
* MgSO4•7H2O 2g | * MgSO4•7H2O 2g | ||
| - | * | + | * Added distilled water to make 1000mL |
| + | * Autoclaved | ||
| - | ''Medium A base'' *10 made up: | + | '''Medium A base''' *10 made up: |
| + | |||
| + | * Yeast extract 10g | ||
| + | * Casamino acids 2g | ||
| + | * Added distilled water to make 900mL | ||
| + | * Autoclaved, then add : | ||
| + | * 50g glucose, filter sterilized 100mL | ||
Revision as of 13:40, 13 July 2012
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Monday
Making bacillus competent
Bacillus salts *10 made up:
- (NH4)2SO4: 20g
- KH2PO4 (anhydrous): 60g
- Anhydrous K2HPO4 189.7g (N.B. Different from the one below!)
- KH2PO4 60g
- Tri-sodium citrate 10g
- MgSO4•7H2O 2g
- Added distilled water to make 1000mL
- Autoclaved
Medium A base *10 made up:
- Yeast extract 10g
- Casamino acids 2g
- Added distilled water to make 900mL
- Autoclaved, then add :
- 50g glucose, filter sterilized 100mL
