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Team:Cambridge/Protocols
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* [[Team:Cambridge/PCRprotocol|PCR using Phusion DNA polymerase]] A method for amplifying a section of DNA. | * [[Team:Cambridge/PCRprotocol|PCR using Phusion DNA polymerase]] A method for amplifying a section of DNA. | ||
* [[Team:Cambridge/PCRcolony|Colony PCR]] PCR with cells as a template. Useful for checking the length of an insert in an introduced plasmid. | * [[Team:Cambridge/PCRcolony|Colony PCR]] PCR with cells as a template. Useful for checking the length of an insert in an introduced plasmid. | ||
| + | * [[Team:Cambridge/GelElectrophoresis|Gel Electrophoresis]] A technique for separating DNA strands of different lengths. | ||
| + | * [[Team:Cambridge/GelExtractionofDNA|Gel Extraction of DNA]] A technique used to isolate a desired fragment of intact DNA from an agarose gel following agarose gel electrophoresis. | ||
* [[Team:Cambridge/Gibsonassembly|Gibson Assembly]] A technique for ligating multiple DNA fragments in one step, compatible with standard assembly. | * [[Team:Cambridge/Gibsonassembly|Gibson Assembly]] A technique for ligating multiple DNA fragments in one step, compatible with standard assembly. | ||
| + | * [[Team:Cambridge/TransformationofB.subtilis|Transformation of ''Bacillus subtilis'']] A technique used to introduce foreign DNA into competent Bacillus cells. | ||
| + | * [[Team:Cambridge/MiniPrep|MiniPrep]] A method used to extract DNA from bacterial cells. | ||
| + | * [[Team:Cambridge/RestrictionDigest| Restriction Enzyme Digest]] A method for creating a restriction map of a plasmid. | ||
| + | * [[Team:Cambridge/SDSPAGE| Protein analysis by SDS PAGE]] A method used to separate polypeptides of different lengths. | ||
'''N.B.''' Information in purple is subject to change through optimisation over the course of our project. | '''N.B.''' Information in purple is subject to change through optimisation over the course of our project. | ||
Revision as of 10:07, 13 July 2012
- PCR using Phusion DNA polymerase A method for amplifying a section of DNA.
- Colony PCR PCR with cells as a template. Useful for checking the length of an insert in an introduced plasmid.
- Gel Electrophoresis A technique for separating DNA strands of different lengths.
- Gel Extraction of DNA A technique used to isolate a desired fragment of intact DNA from an agarose gel following agarose gel electrophoresis.
- Gibson Assembly A technique for ligating multiple DNA fragments in one step, compatible with standard assembly.
- Transformation of Bacillus subtilis A technique used to introduce foreign DNA into competent Bacillus cells.
- MiniPrep A method used to extract DNA from bacterial cells.
- Restriction Enzyme Digest A method for creating a restriction map of a plasmid.
- Protein analysis by SDS PAGE A method used to separate polypeptides of different lengths.
N.B. Information in purple is subject to change through optimisation over the course of our project.
