"
Team:Purdue/Characterization
From 2012.igem.org
(Difference between revisions)
| Line 30: | Line 30: | ||
A powerpoint highlighting possible characterization methods is given <a href="https://static.igem.org/mediawiki/2012/8/8e/Experiments_for_Silica_Characterization.ppt">here</a> | A powerpoint highlighting possible characterization methods is given <a href="https://static.igem.org/mediawiki/2012/8/8e/Experiments_for_Silica_Characterization.ppt">here</a> | ||
</br> | </br> | ||
| - | After discussion with the team and our faculty advisor, Dr. Rickus, we have decided to use the scanning eletron microscopy (SEM), x-ray diffraction (XRD), and a live/dead assay ( | + | After discussion with the team and our faculty advisor, Dr. Rickus, we have decided to use the scanning eletron microscopy (SEM), x-ray diffraction (XRD), and a live/dead assay (we plan to use <a href="http://products.invitrogen.com/ivgn/product/L7012?ICID=search-product">Baclight Bacterial Viability Kit</a>). |
</html> | </html> | ||
Revision as of 18:27, 12 July 2012
Characterization & Experimental Design
Meeting 7/2/12
List of What Needs to get Done this Week:- Research protocols on setting up and running experiments using biofilms
- Research flow and static protocols for biofilms
- Research ways to characterize silica matrix and acquire quantifiable data
- Create timelines for each assay researched
- Order reagents and get training on any equipment needed for assays
Silica Characterization
Research was done on different ways to characterize the silica coat. Three extremely helpful articles are listed below.- Cell-Mediated Deposition of Porous Silica on Bacterial Biofilms
- Sol-gel synthsis and structure of silica hybrid materials
- Thin-film silica sol-gel coatings for neural microelectrodes
