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Team:Cambridge/Protocols
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| - | * [[Team:Cambridge/PCRprotocol|PCR using Phusion DNA polymerase]] | + | * [[Team:Cambridge/PCRprotocol|PCR using Phusion DNA polymerase]] A method for amplifying a section of DNA. |
| - | * [[Team:Cambridge/PCRcolony|Colony PCR]] | + | * [[Team:Cambridge/PCRcolony|Colony PCR]] PCR with cells as a template. Useful for checking the length of an insert in an introduced plasmid. |
| - | * [[Team:Cambridge/Gibsonassembly|Gibson Assembly]] | + | * [[Team:Cambridge/Gibsonassembly|Gibson Assembly]] A technique for ligating multiple DNA fragments in one step, compatible with standard assembly. |
'''N.B.''' Information in purple is subject to change through optimisation over the course of our project. | '''N.B.''' Information in purple is subject to change through optimisation over the course of our project. | ||
Revision as of 13:17, 12 July 2012
- PCR using Phusion DNA polymerase A method for amplifying a section of DNA.
- Colony PCR PCR with cells as a template. Useful for checking the length of an insert in an introduced plasmid.
- Gibson Assembly A technique for ligating multiple DNA fragments in one step, compatible with standard assembly.
N.B. Information in purple is subject to change through optimisation over the course of our project.
