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Team:Cornell/Notebook/Salicylate reporter/July8
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| - | Dylan performed a Phusion PCR with a single annealing temperature of 59 degrees Celsius using primers 27 and 28 for the final time. Should this PCR be unsuccessful, new primers will be designed. The purpose of this PCR was to amplify [[Team:Cornell/Notebook/StrainList|p21]]. The PCR product was analyzed using agarose gel electrophoresis to determine if the desired 1.127 kb fragment is present. | + | Dylan performed a [[Team:Cornell/Notebook/Phusion_PCR|Phusion PCR]] with a single annealing temperature of 59 degrees Celsius using primers 27 and 28 for the final time. Should this PCR be unsuccessful, new primers will be designed. The purpose of this PCR was to amplify [[Team:Cornell/Notebook/StrainList|p21]]. The PCR product was analyzed using agarose gel electrophoresis to determine if the desired 1.127 kb fragment is present. |
To transform our plasmids into Shewanella with conjugation, we had asked Dr. Jeff Gralnick for WM3064 E. coli for which plates containing 2,6-Diaminopimelic Acid (DAP) are required. In preparation to receive this strain, the plates with DAP were prepared. | To transform our plasmids into Shewanella with conjugation, we had asked Dr. Jeff Gralnick for WM3064 E. coli for which plates containing 2,6-Diaminopimelic Acid (DAP) are required. In preparation to receive this strain, the plates with DAP were prepared. | ||
[[Image:2012_7_9_FearlessLeaderAkaLordOfFlame.jpg|thumb|right|Claire spark-lighting a Bunsen burner.]] | [[Image:2012_7_9_FearlessLeaderAkaLordOfFlame.jpg|thumb|right|Claire spark-lighting a Bunsen burner.]] | ||
Revision as of 16:10, 11 July 2012
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The salicylate reporter is analogous to the arsenic reporter, but with a salicylate-sensitive promoter. That is, in the presence of salicylate it will produce MtrB, completing the Mtr pathway and allowing Shewanella to produce current in our biosensor. In combination with the nah operon, this reporter will be able to detect naphthalene levels. Back to salicylate reporter week view
July 8th-14th
July 9th
Dylan performed a Phusion PCR with a single annealing temperature of 59 degrees Celsius using primers 27 and 28 for the final time. Should this PCR be unsuccessful, new primers will be designed. The purpose of this PCR was to amplify p21. The PCR product was analyzed using agarose gel electrophoresis to determine if the desired 1.127 kb fragment is present. To transform our plasmids into Shewanella with conjugation, we had asked Dr. Jeff Gralnick for WM3064 E. coli for which plates containing 2,6-Diaminopimelic Acid (DAP) are required. In preparation to receive this strain, the plates with DAP were prepared.
July 10th
Caleb miniprepped strains P8, P9, P10, P15, and P16 (See strain list)
==July 11th==
