Team:Lyon-INSA/BiofilmK

From 2012.igem.org

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<p><OL><li><strong>KILL and SCATTER</strong> the biofillm. The effect of the biocide and scattering agents produced by the bacteria can be enhanced by the “torpedo” behavior of the <i>Bacillus</i> swimmers.
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<p><OL><li><strong>KILL and SCATTER</strong> the biofilm. The effect of the biocide and scattering agents produced by the bacteria can be enhanced by the “torpedo” behavior of the <i>Bacillus</i> swimmers.
</br><li><strong>COAT</strong> the surface with a surfactant reagent in presence of the inducteur 1 (xylose).  
</br><li><strong>COAT</strong> the surface with a surfactant reagent in presence of the inducteur 1 (xylose).  
</br><li><strong>STICK</strong> to install a positif biofilm in presence of the inducteur 2 (IPTG).
</br><li><strong>STICK</strong> to install a positif biofilm in presence of the inducteur 2 (IPTG).
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<br>Such estimations made us confident on the competitive potential of our bacteria, considering the low cost of their growth. The only thing left to be done was then to prove it by estimating the cost of our own solution.
<br>Such estimations made us confident on the competitive potential of our bacteria, considering the low cost of their growth. The only thing left to be done was then to prove it by estimating the cost of our own solution.
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   <h1> Biofilm Killer implementation</h1>
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   <h1> Biofilm Killer implementation : a viable solution</h1>
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We had a difficult time evaluating the potential economic value of our product, but we eventually made it.
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<center><img src="https://static.igem.org/mediawiki/2012/a/a8/BK_implementation.PNG"/></center>
<center><img src="https://static.igem.org/mediawiki/2012/a/a8/BK_implementation.PNG"/></center>
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   <h1>Main advantages</h1>
   <h1>Main advantages</h1>

Revision as of 20:33, 26 October 2012

The INSA-Lyon iGEM 2012 solution: “Biofilm Killer”


Biological solutions presented above are very interesting and promising. But synthetic biology could make them even more powerful by providing a technology:

  • able to control, prevent and protect industrial equipment
  • using non-persistent molecules in the environment
  • minimizing harm to individual, products and environment


To meet these challenges, we have chosen to build a bacterial strain based on the environmental friendly Bacillus subtilis strain (already used to feed animals and promote healthy vegetable growth. “Biofilm Killer” was engineered to both destroy and then replace, if needed, the deleterious contamination by a positive biofilm.
Our solution is based on 3 genetic modules:

  1. KILL and SCATTER the biofilm. The effect of the biocide and scattering agents produced by the bacteria can be enhanced by the “torpedo” behavior of the Bacillus swimmers.
  2. COAT the surface with a surfactant reagent in presence of the inducteur 1 (xylose).
  3. STICK to install a positif biofilm in presence of the inducteur 2 (IPTG).

Estimating the cost of the current industrial solutions

In order to compare our biological solution to the currently used ones, we first approached companies whose concerns were similar to ours : they kindly gave us some of their data related to the price of their sterilizing solution.

A first company that accept to answer our questions estimated that, considering the use of chemical reagents (€50 per use : 3 kg of cleaning products, €15 of disinfectants) and of a sterilizing filter to recover biological debris (€100 to €300 per use depending on its size), the total cost of a 4 hour wash of a 1000L tank was from €165 to €365 ($215 to $475)per sterilization.

The second one indicated a €480 ($625) cost estimation for a 1500L tank per sterilization.


Such estimations made us confident on the competitive potential of our bacteria, considering the low cost of their growth. The only thing left to be done was then to prove it by estimating the cost of our own solution.

Biofilm Killer implementation : a viable solution


We had a difficult time evaluating the potential economic value of our product, but we eventually made it.

Main advantages

Lower cost : No need to have a protein purification step which is very expensive.
Eco-friendly: “Biofilm Killer” won’t release toxic chemicals in the environment since the proteins used can be easily destroyed.
The “biological swiss-knife”: “Biofilm Killer” should be able to replace mechanical cleaning actions thanks to the swarming properties of our strain which can penetrate deep inside the biofilm and release the active molecules in situ all over the biofilm.

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