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Team:LMU-Munich/Data/cgeA
From 2012.igem.org
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| - | <p align="justify">cgeA turned out to contain an ''Age''I site. After mutating that, the ''Not''I from the prefix was dysfunctional due to the insertion of one nucleotide. After putting so much effort into such a small gene, it refused to be combined with any of our promoters, not to | + | <p align="justify">cgeA turned out to contain an ''Age''I site. After mutating that, the ''Not''I from the prefix was dysfunctional due to the insertion of one nucleotide. After putting so much effort into such a small gene, it refused to be combined with any of our promoters, not to mention gene fusions with any of our reporter genes.</p> |
<p align="justify">It should have been obvious that P<sub>''cgeA''</sub> is not better than the gene it regulates. It was not easy to clone this promoter into pSB<sub>''Bs''</sub>3C-<i>lux</i>ABCDE, but when it was finally transformed into ''B. subtilis'', it revealed no activity.</p> | <p align="justify">It should have been obvious that P<sub>''cgeA''</sub> is not better than the gene it regulates. It was not easy to clone this promoter into pSB<sub>''Bs''</sub>3C-<i>lux</i>ABCDE, but when it was finally transformed into ''B. subtilis'', it revealed no activity.</p> | ||
Revision as of 13:42, 26 October 2012
The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".
[ more news ]
cgeA and PcgeA
cgeA turned out to contain an AgeI site. After mutating that, the NotI from the prefix was dysfunctional due to the insertion of one nucleotide. After putting so much effort into such a small gene, it refused to be combined with any of our promoters, not to mention gene fusions with any of our reporter genes.
It should have been obvious that PcgeA is not better than the gene it regulates. It was not easy to clone this promoter into pSBBs3C-luxABCDE, but when it was finally transformed into B. subtilis, it revealed no activity.
It's needless to say, that of cours we had the cobination of cgeA with exactly that non-functional promoter.
To complete our confusion, the promoter still seemed to work in combination with the Sporobead construction. Weak, but worked....
