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Team:Potsdam Bioware/Lab/Labjournal/October
From 2012.igem.org
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| + | <p style="background-color: rgb(238, 221, 130); font-weight:bold;">cell culture </p> | ||
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| - | + | <b>Investigator:</b>Kerstin<br> | |
| - | <b>Investigator:</b> | + | |
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| - | + | <b>Topic: </b>cell-culture <br> | |
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| - | + | * passaging of HT1080 | |
| - | + | * dilution of stably transfected cells (Nanobody construct clone 29, originally from transfected Well 3) to 1-2 cells per 150µl and seeding in 4x 96-Well plates | |
| - | + | * seeding of stably transfected CHO Clone 29 cells in Ibidi Dish | |
| - | + | * change of medium in 75cm² flasks with stably transfected cells (Clone 4 and 29) | |
| - | + | * harvest cells for Western Blot (CHO not transfected, CHO stably transfected Clone 4 and 29) | |
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Revision as of 13:36, 25 October 2012
Contents |
AID
2012-10-10
Inoculation of plasmid samples of the 48h retransformation plates (after FACS)
Investigators: Tom
Time: 2012-10-10
Materials:
- LB medium
- Amp stock solution
- plate with cultures:
EGFR-C-AID without NES, with NLS+Kozak sequence+eGFP
(after FACS selection)
Method:
Inoculation of:
5 cultures per plate in 5 ml LB medium + 5µL Amp.
(--> 5 cultures)
Further tasks:
- Miniprep
Antibody
12.10.2012
cell culture
Investigator:Kerstin
Topic: cell-culture
- passaging of HT1080
- dilution of stably transfected cells (Nanobody construct clone 29, originally from transfected Well 3) to 1-2 cells per 150µl and seeding in 4x 96-Well plates
- seeding of stably transfected CHO Clone 29 cells in Ibidi Dish
- change of medium in 75cm² flasks with stably transfected cells (Clone 4 and 29)
- harvest cells for Western Blot (CHO not transfected, CHO stably transfected Clone 4 and 29)
Virus
XXX
XXX
Investigator: XXX
Aim: XXX
Materials:
XXX
Method:
XXX
Results:
XXX
Further tasks
XXX
