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Team:Groningen/Notebook/Modeling 9July2012
From 2012.igem.org
(Created page with "Multihost vector (BBa_I742123) double digestion with EcoRI-SpeI and XbaI-PstI. | Result: Gel image of this vector is inconsistent. BBa_I742123 was isolated from 3 different E. co...") |
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Multihost vector (BBa_I742123) double digestion with EcoRI-SpeI and XbaI-PstI. | Multihost vector (BBa_I742123) double digestion with EcoRI-SpeI and XbaI-PstI. | ||
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Revision as of 13:17, 9 July 2012
(AP) Multihost vector (BBa_I742123) double digestion with EcoRI-SpeI and XbaI-PstI. | Result: Gel image of this vector is inconsistent. BBa_I742123 was isolated from 3 different E. coli colonies and was double-digested. The result of this reaction, however, varies between isolates (gel picture will be updated soon). | | PCR of alsT promoter region using -150, -250, -300, -500 forward primer. | Last week the annealing temperature of each primer sets has been optimized. We experienced the best result in 58-60oC. Today the PCR was done with different polymerase, Pfu, instead of Taq. Annealing temperature used in this PCR was 60oC. | Result: Soon updated | | Biobrick parts that was requested from iGEM HQ finally arrived!! | List of biobrick parts: 1. BBa_K116603 2. BBa_K116602 3. BBa_J37019 4. BBa_K116639 5. BBa_K116609 | E. coli containing these parts were inoculated into LB agar+100ug/ml Ampicilin. Incubate overnight in 37oC.
