Team:LMU-Munich/Bacillus BioBricks

From 2012.igem.org

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<p align="justify">Since ''Bacillus subtilis'' is not an organism commonly used in iGEM, please check out our [https://2012.igem.org/Team:LMU-Munich/Bacillus_Introduction Introduction].</p>
<p align="justify">Since ''Bacillus subtilis'' is not an organism commonly used in iGEM, please check out our [https://2012.igem.org/Team:LMU-Munich/Bacillus_Introduction Introduction].</p>
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<div class="box">
==''Bacillus'' Vectors  [[File:LMU Backbone.png|100px|link=Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Vectors|Vectors]]==
==''Bacillus'' Vectors  [[File:LMU Backbone.png|100px|link=Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Vectors|Vectors]]==
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{| "width=100%" style="text-align:center;" style="align:right"|
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|<p align="justify">We have generated a suit of BioBrick-compatible vectors: three empty insertional backbones with different antibiotic resistances and integration loci, two reporter and two expression vectors. Here is a list of all the vectors we cloned and used.</p>  
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<p align="justify">We have generated a suit of BioBrick-compatible vectors: three empty insertional backbones with different antibiotic resistances and integration loci, two reporter and two expression vectors. Here is a list of all the vectors we cloned and used.</p>  
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|[[File:LMU-Munich-PSBBs1C.png|200px|right|link=Team:LMU-Munich/Bacillus BioBricks/Vectors]]
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{| class="colored"
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|-
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!Vector Name
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!colspan="2"|Resistance
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!Insertion
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!Description
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!colspan="2"|Vector origin
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!<font color="#EBFCE4" size="2">BioBrick</font>
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!''Eco''
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!''Bsu''
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!locus
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!
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!Name
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!Reference
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!pSB<sub>''Bs''</sub>1C [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823023 <font color="#EBFCE4" size="2">(BBa_K823023)</font>]
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|Amp
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|Cm
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|''amyE''
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|empty
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|pDG1662
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|[http://www.ncbi.nlm.nih.gov/pubmed/8973347 Guérout-Fleury]
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|-
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!pSB<sub>''Bs''</sub>4S [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823022 <font color="#EBFCE4" size="2">(BBa_K823022)</font>]
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|Amp
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|Spec
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|''thrC''
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|empty
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|pDG1731
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|[http://www.ncbi.nlm.nih.gov/pubmed/8973347 Guérout-Fleury]
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|-
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!pSB<sub>''Bs''</sub>2E [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823027 <font color="#EBFCE4" size="2">(BBa_K823027)</font>]
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|Amp
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|MLS
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|''lacA''
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|empty
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|pAX01
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|[http://www.ncbi.nlm.nih.gov/pubmed/11274134 Härtl]
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|-
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!pSB<sub>''Bs''</sub>1C-<i>lac</i>Z [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823021 <font color="#EBFCE4" size="2">(BBa_K823021) </font>]
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|Amp
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|Cm
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|''amyE''
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|<i>lacZ</i> reporter
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|pAC6
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|[http://www.ncbi.nlm.nih.gov/pubmed/11902727 Stülke]
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|-
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!pSB<sub>''Bs''</sub>3C-<i>lux</i>ABCDE [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823025 <font color="#EBFCE4" size="2">(BBa_K823025)</font>]
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|Amp
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|Cm
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|''sacA''
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|<i>luxABCDE</i> reporter
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|pAH328
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|[http://www.ncbi.nlm.nih.gov/pubmed/20709900 Schmalisch]
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!pSB<sub>''Bs''</sub>4S-P<sub><i>Xyl</i></sub> [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823024 <font color="#EBFCE4" size="2">(BBa_K823024)</font>]
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|Amp
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|Spec
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|''thrC''
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|Xylose-promoter
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|pXT
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|[http://www.ncbi.nlm.nih.gov/pubmed/11069659 Derré]
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|-
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!pSB<sub>''Bs''</sub>0K-P<sub><i>spac</i></sub> [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823026 <font color="#EBFCE4" size="2">(BBa_K823026)</font>]
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|Amp
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|Kan
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|replicative
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|IPTG-promoter
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|pDG148
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|[http://www.ncbi.nlm.nih.gov/pubmed/11728721 Joseph]
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!'''Sporo'''vector [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823054 <font color="#EBFCE4" size="2">(BBa_K823054)</font>]
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|Amp
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|Spec
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|''thrC''
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|to create [https://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins '''Sporo'''beads]
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|pSB<sub>''Bs''</sub>4S
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|[[Team:LMU-Munich/Bacillus_BioBricks/Sporovector|'''Sporo'''vector]]
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|-
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|}
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<br>
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<p align="justify">For the use of our vectors please see our [[Team:LMU-Munich/Lab_Notebook/Protocols|Protocols]] page. A general introduction to [[Team:LMU-Munich/Bacillus Introduction |''Bacillus subtilis'']] and its integrative vectors can be found [[Team:LMU-Munich/Bacillus_BioBricks/integration|here]]. All vectors have ampicillin as <i>Escherichia coli</i> resistance and RFP in the multiple cloning site as selection marker.</p>
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The corresponding vector maps:
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{|
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|<html> <a> <img src="https://static.igem.org/mediawiki/2012/e/e0/LMU-Munich-PSBBs1C.png" height=130"/></a></html>
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|<html> <a> <img src="https://static.igem.org/mediawiki/2012/2/2a/LMU-Munich-PSBBs4S.png" height=130"/></a></html>
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|-
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|<html> <a> <img src="https://static.igem.org/mediawiki/2012/4/46/LMU-Munich-PSBBs2E.png" height=130"/></a></html>
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|<html> <a> <img src="https://static.igem.org/mediawiki/2012/0/04/LMU-Munich-PSBBs1C-lacZ.png" height=130"/></a></html>
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|-
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|<html> <a> <img src="https://static.igem.org/mediawiki/2012/f/fc/LMU-Munich-PSBBs3C-luxABCDE.png" height=130"/></a></html>
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|<html> <a> <img src="https://static.igem.org/mediawiki/2012/d/de/LMU-Munich-PSBBs4S-Pxyl.png" height=130"/></a></html>
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|-
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|<html> <a> <img src="https://static.igem.org/mediawiki/2012/0/03/LMU-Munich-PSBBs0K-Pspac.png" height=130"/></a></html>
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|<html> <a> <img src="https://static.igem.org/mediawiki/2012/a/ab/LMU-Munich-Sporovector.png" height=130"/></a></html>
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|-
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|}
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<p align="justify">The number in the vector's name codes for the insertion locus and the following letter for the <i> Bacillus subtilis </i> resistance gene according to the following table:</p>
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{| class="colored"
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|-
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!Number
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!Insertion locus
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!Letter
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!Resistance
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|-
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!0
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|replicative
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!C
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|Chloramphenicol (Cm)
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|-
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!1
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|''amyE'' (amylase)
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!E
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|MLS (Erythromycin + Lincomycin)
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!2
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|''lacA'' (β-galactosidase)
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!K
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|Kanamycin (Kan)
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!3
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|''sacA'' (sucrase)
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!S
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|Spectinomycin (Spec)
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!4
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|''thrC'' (threonine synthase)
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|-
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! colspan="2" |[[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Bacillus BioBricks/Vectors]]
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|}
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</div>
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<p align="justify">The concentrations of the antibiotics for selection of ''B.subtilis'' transformation and tests for plasmid insertion can be found in our [https://2012.igem.org/Team:LMU-Munich/Lab_Notebook/Protocols Protocol] section.</p>  
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<p align="justify">See [[Team:LMU-Munich/Bacillus_BioBricks/vector_use| here]] to find out how to use ''B. subtilis'' vectors. In this [[Team:LMU-Munich/Bacillus_BioBricks/integration|overview]], the integration mechanism of ''B. subtilis'' vectors is described.</p>
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<p align="justify">The design and special use of our Sporovector can be found [[Team:LMU-Munich/Bacillus_BioBricks/Sporovector |here]].</p>
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For results, check out our Data page: [[File:LMU Arrow purple.png|40px|link=Team:LMU-Munich/Data/Vectors]]
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Revision as of 20:41, 24 October 2012

iGEM Ludwig-Maximilians-Universität München Beadzillus

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The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

IGEM HQ LMU prize.jpg

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