"
Team:LMU-Munich/Data/crustpromoters
From 2012.igem.org
Franzi.Duerr (Talk | contribs) |
|||
| Line 30: | Line 30: | ||
<p align="justify">The native promoters of ''cotZ'', P<sub>''cotYZ''</sub> and P<sub>''cotV''</sub>, show a clear signal late in stationary phase, as expected. P<sub>''cotYZ''</sub> is activated two hours earlier than P<sub>''cotV''</sub> and both promoters are active for about 12 hours. In contrast, P<sub>''cgeA''</sub> does not show any activity. </p> | <p align="justify">The native promoters of ''cotZ'', P<sub>''cotYZ''</sub> and P<sub>''cotV''</sub>, show a clear signal late in stationary phase, as expected. P<sub>''cotYZ''</sub> is activated two hours earlier than P<sub>''cotV''</sub> and both promoters are active for about 12 hours. In contrast, P<sub>''cgeA''</sub> does not show any activity. </p> | ||
| + | [[File:Arrow purple Data.png|center|100px|link=Team:LMU-Munich/Data]] | ||
| - | |||
{{:Team:LMU-Munich/Templates/Page Footer}} | {{:Team:LMU-Munich/Templates/Page Footer}} | ||
Revision as of 20:44, 23 October 2012
The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".
[ more news ]
Crust promoter evaluation
The figure below shows the result of the evaluation of the three promoters (PcgeA, PcotV, PcotYZ) for the spore crust proteins with a previous version of the luminescence reporter vector [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823025 pSBBs3C-luxABCDE]. For each promoter two independent clones were evaluated three times. To link the promoter activities to the growth phase, the optical density (OD600) was measured throughout the whole experiment.
|
The native promoters of cotZ, PcotYZ and PcotV, show a clear signal late in stationary phase, as expected. PcotYZ is activated two hours earlier than PcotV and both promoters are active for about 12 hours. In contrast, PcgeA does not show any activity.
