"
Team:LMU-Munich/Spore Coat Proteins/result
From 2012.igem.org
(Created page with "{{:Team:LMU-Munich/Templates/Page Header|File:Team-LMU_Photo2.jpg}} link=Team:LMU-Munich/Spore_Coat_Proteins ====Main Results==== <p...")
Newer edit →
Revision as of 19:50, 23 October 2012
The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".
[ more news ]
Main Results
We were able to finish five constructs and integrated them into wild type W168 and the ΔcotZ mutant:
| recipient strain W168 | recipient strain B 49 (W168 ΔcotZ) | |
|---|---|---|
| pSBBs1C-PcotYZ-cotZ-2aa-gfp-terminator | B 53 | B 70 |
| pSBBs1C-PcotYZ-cotZ-gfp-terminator | B 54 | B 71 |
| pSBBs1C-PcotV-cotZ-2aa-terminator | B 55 | B 72 |
| pSBBs1C-PcotV-cotZ-terminator | B 56 | B 73 |
| pSBBs1C-PcgeA-cotZ-2aa-terminator | B 52 | B 69 |
Finally, we started with the most important experiment for our GFP-Sporobeads, the fluorescence microscopy. We developed a sporulation protocol (for details see Protocol for enhancement of mature spore numbers) that increases the rates of mature spores in our samples. The cells were fixed on agarose pads and investigated by phase contrast and fluorescence microscopy. While spores of the wild type only showed the known background fluorescence, all Sporobeads showed bright green fluorescence at the edge (on the surface) of the spores. Sporobeads from strain B 53 (containing the PcotYZ-cotZ-2aa-gfp-terminator construct) showed the highest fluorescence intensity (see Fig. 5 and all data). Hence, this strain was chosen for further experiments.
|
