Team:Potsdam Bioware/Lab/Labjournal/October

From 2012.igem.org

(Difference between revisions)
(→2012-09-02)
(→AID)
Line 7: Line 7:
===<p style="background-color: rgb(240, 20, 70);">XXX</p>===
===<p style="background-color: rgb(240, 20, 70);">XXX</p>===
-
<p style="background-color: rgb(238, 221, 130); font-weight: bold;">XXX</p>
+
<p style="background-color: rgb(238, 221, 130); font-weight: bold;"> Inoculation of plasmid samples of the 48h retransformation plates (after FACS)</p>
-
<Br>
+
<b>Investigators:</b> Tom
-
<b>Investigators:</b><Br>
+
<br>
-
XXX
+
<b>Time:</b> 2012-10-10 <br>
-
<Br>
+
<br>
-
<b>Aim:</b><Br>
+
<b>Materials:</b><br>
-
XXX
+
* LB medium <br>
-
<b>Method:</b><Br>
+
* Amp stock solution<br>
-
XXX
+
*plate with cultures: <br>
-
<Br>
+
EGFR-C-AID without NES, with NLS+Kozak sequence+eGFP<br>
-
<b>Results:</b><Br>
+
(after FACS selection)<br>
-
XXX<Br>
+
<b>Method:</b><br>
 +
 
 +
Inoculation of:
 +
 
 +
5 cultures per plate in 5 ml LB medium + 5µL Amp.
 +
 
 +
(--> 5 cultures)<br>
 +
 
 +
<b>Further tasks:</b><br>
 +
 
 +
* Miniprep <br>
<Br>
<Br>

Revision as of 09:35, 22 October 2012


Contents

AID

XXX

Inoculation of plasmid samples of the 48h retransformation plates (after FACS)

Investigators: Tom


Time: 2012-10-10


Materials:

  • LB medium
  • Amp stock solution
  • plate with cultures:

EGFR-C-AID without NES, with NLS+Kozak sequence+eGFP

(after FACS selection)

Method:

Inoculation of:

5 cultures per plate in 5 ml LB medium + 5µL Amp.

(--> 5 cultures)

Further tasks:

  • Miniprep


Antibody

XXX

XXX


Investigator:XXX


Time: XXX


Materials: XXX


Methods: XXX


Results:

XXX


Further tasks: XXX


Virus

XXX

XXX

Investigator: XXX

Aim: XXX

Materials:

XXX

Method:

XXX

Results:

XXX

Further tasks

XXX
Retrieved from "http://2012.igem.org/Team:Potsdam_Bioware/Lab/Labjournal/October"