Team:Potsdam Bioware/Lab/Labjournal/October

From 2012.igem.org

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==Virus==
==Virus==
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===<p style="background-color: rgb(240, 20, 70);"> 2012-09-02</p>===
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===<p style="background-color: rgb(240, 20, 70);"> XXX</p>===
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<p style="background-color: rgb(238, 221, 130); font-weight: bold;"> Topic: preparing viral stocks</p>
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<p style="background-color: rgb(238, 221, 130); font-weight: bold;"> XXX</p>
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<b>Investigator: </b> Kathi <br>
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<b>Investigator: </b> XXX<br>
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<b> Aim: </b> preparation of the primary virus stock<br>
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<b> Aim: </b> XXX<br>
<b>Materials:</b><br>
<b>Materials:</b><br>
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* cell-culture --> HEK AAV 293 transfected cells on 29.08.2012 (with following plasmids: p01, p06, phelper, psb1c3)
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XXX
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* 80 °C freezer
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* 37 °C water bath <br>
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<b>Method:</b><br>
<b>Method:</b><br>
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* transfer transfected cells (incl. DMEM) to a falcon tube
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XXX
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* 4 times: 10 min freeze the falcon tube (-80 °C) and 15 min unfreezing (37 °C water bath)
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* centrifugation (10 min, 10000 '''g''', RT)
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* supernatant = virus stock
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* sore at -80 °C <br>
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<b>Results:</b><br>
<b>Results:</b><br>
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* virus stock → with YFP on the surface and GOI = CFP <br>
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XXX
<b>Further tasks</b>
<b>Further tasks</b>
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* infection
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XXX

Revision as of 09:28, 22 October 2012


Contents

AID

XXX

XXX


Investigators:

XXX


Aim:

XXX

Method:

XXX


Results:

XXX


Antibody

XXX

XXX


Investigator:XXX


Time: XXX


Materials: XXX


Methods: XXX


Results:

XXX


Further tasks: XXX


Virus

XXX

XXX

Investigator: XXX

Aim: XXX

Materials:

XXX

Method:

XXX

Results:

XXX

Further tasks

XXX
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