Team:Potsdam Bioware/Lab/Labjournal/October

From 2012.igem.org

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==AID==
==AID==
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===<p style="background-color: rgb(240, 20, 70);">2012-09-03</p>===
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===<p style="background-color: rgb(240, 20, 70);">XXX</p>===
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<p style="background-color: rgb(238, 221, 130); font-weight: bold;">Miniprep of mVENUS construct and wildtype AID from transfected CHO cells</p>
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<p style="background-color: rgb(238, 221, 130); font-weight: bold;">XXX</p>
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<Br>
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<b>Investigators:</b><Br>
<b>Investigators:</b><Br>
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Rico
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XXX
<Br>
<Br>
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<b>Aim:</b><Br>
<b>Aim:</b><Br>
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*Miniprep of YFP-construct and wildtype AID from transfected CHO cells to proof whether it is possible to transform E.coli with the purified construct for sequencing
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XXX
<b>Method:</b><Br>
<b>Method:</b><Br>
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*Miniprep of cells
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XXX
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*Elution with 50 µL
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<Br>
<Br>
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<b>Results:</b><Br>
<b>Results:</b><Br>
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CHO transfected with YFP WT-AID = 2.8 ng/µL<Br>
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XXX<Br>
<Br>
<Br>
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==Antibody==
==Antibody==

Revision as of 09:26, 22 October 2012


Contents

AID

XXX

XXX


Investigators:

XXX


Aim:

XXX

Method:

XXX


Results:

XXX


Antibody

2012-09-01

Glycerol stock and Mini Prep of Geneart construct, Venus, pAK100+scFv425 R H1, R H2, R L1, R L2, L1, L2


Investigator:Maria


Time: 2012-09-01 9:30 am


Materials: overnight cultures Geneart construct (4x), Venus (Tom), pAK100+scFv425 R H1, R H2, R L1, R L2, L1, L2 (Chris), Mini prep kit


Methods: according to manual


Results:

Venus: …. ng/µl

pAK100+scFv425 R H1: --- ng/µl

pAK100+scFv425 R H2: --- ng/µl<br<

pAK100+scFv425 R L1: --- ng/µl

pAK100+scFv425 R L2: --- ng/µl

pAK100+scFv425 L1: --- ng/µl

pAK100+scFv425 L2: --- ng/µl

Geneart construct 1: --- ng/µl

Geneart construct 2: --- ng/µl

Geneart construct 3: --- ng/µl

Geneart construct 4: --- ng/µl

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Glycerole stocks of Geneart construct and pAK100+scFv425 R H1, R H2, R L1, R L2, L1, L2


Further tasks:



Virus

2012-09-02

Topic: preparing viral stocks

Investigator: Kathi

Aim: preparation of the primary virus stock

Materials:

  • cell-culture --> HEK AAV 293 transfected cells on 29.08.2012 (with following plasmids: p01, p06, phelper, psb1c3)
  • 80 °C freezer
  • 37 °C water bath

Method:

  • transfer transfected cells (incl. DMEM) to a falcon tube
  • 4 times: 10 min freeze the falcon tube (-80 °C) and 15 min unfreezing (37 °C water bath)
  • centrifugation (10 min, 10000 g, RT)
  • supernatant = virus stock
  • sore at -80 °C

Results:

  • virus stock → with YFP on the surface and GOI = CFP

Further tasks

  • infection
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