Team:Wageningen UR/Journal/week22
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(Created page with "{{Template: WUR}} = week 22: 24 September - 30 September = '''GFP modification''' ---- ''24 September'' * miniprep of GFP-coil in Bba_J04500, GFP-coil with His tag in Bba_J0...")
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(Created page with "{{Template: WUR}} = week 22: 24 September - 30 September = '''GFP modification''' ---- ''24 September'' * miniprep of GFP-coil in Bba_J04500, GFP-coil with His tag in Bba_J0...")
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Revision as of 09:03, 22 October 2012
week 22: 24 September - 30 September
GFP modification
24 September
- miniprep of GFP-coil in Bba_J04500, GFP-coil with His tag in Bba_J04500, GFP-coil in pSB1C3 and GFP-coil with His tag in pSB1C3
- sending the minipreps to the parts registry
- Growing and inducing the GFP-coil in Bba_J04500 as well as the GFP-coil with His tag in Bba_J04500 and induced it with IPTG at an OD of ~0.6
-> no green fluorescence could be seen