Team/CINVESTAV-IPN-UNAM MX/home.htm

From 2012.igem.org

(Difference between revisions)
Line 1: Line 1:
-
<html xmlns="http://www.w3.org/1999/xhtml"><head>
+
<html xmlns="http://www.w3.org/1999/xhtml">
-
<title>Home</title>
+
<head>
 +
<meta http-equiv="content-type" content="text/html; charset=utf-8" />
 +
<title>Rho</title>
-
<link rel="stylesheet" href="https://dl.dropbox.com/u/199422/Cinvestav/style.css" />
+
<link href="http://dl.dropbox.com/u/25144539/estilopsu6.cs" rel="stylesheet" type="text/css" />
 +
<meta name="keywords" content="" />
 +
<meta name="description" content="" />
 +
<style type="text/css">
 +
* {
 +
margin: 0;
 +
padding: 0;
 +
}
 +
body {
 +
background: #FFFFFF url(https://static.igem.org/mediawiki/2012/1/14/Img01.gif) repeat-x;
 +
text-align: justify;
 +
font-family: Georgia, "Times New Roman", Times, serif;
 +
font-size: 14px;
 +
color: #000000;
 +
}
-
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
+
h1, h2, h3 {
-
<style type="text/css">td img {display: block;}</style>
+
color: #333333;
 +
}
 +
 
 +
h1, h2 {
 +
margin-bottom: 20px;
 +
font-weight: normal;
 +
}
 +
 
 +
h1 {
 +
font-size: 197%;
 +
}
 +
 
 +
h2 {
 +
font-size: 152%;
 +
}
 +
 
 +
h3 {
 +
font-size: 100%;
 +
}
 +
 
 +
p, ul, ol {
 +
margin-bottom: 20px;
 +
line-height: 1.8em;
 +
}
 +
 
 +
ul, ol {
 +
margin-left: 3em;
 +
}
 +
 
 +
blockquote {
 +
padding-left: 3em;
 +
background: url(https://static.igem.org/mediawiki/2012/1/11/Img08.gif) no-repeat;
 +
font-style: italic;
 +
}
 +
 
 +
a:link {
 +
color: #1953A7;
 +
}
 +
 
 +
a:active {
 +
color: #ED5C0C;
 +
}
 +
 
 +
a:visited {
 +
color: #666666;
 +
}
 +
 
 +
a:hover {
 +
text-decoration: none;
 +
color: #067600;
 +
}
 +
 
 +
img {
 +
border: none;
 +
}
 +
 
 +
img.left {
 +
float: left;
 +
margin: 0 20px 10px 0;
 +
}
 +
 
 +
#menu {
 +
width: 770px;
 +
height: 65px;
 +
margin: 0 auto;
 +
border-right: 1px solid #EDEBD5;
 +
}
 +
 
 +
#menu ul {
 +
margin: 0;
 +
padding: 0;
 +
list-style: none;
 +
}
 +
 
 +
#menu li {
 +
display: inline;
 +
font-size: 12px;
 +
color:#666666
 +
}
 +
 
 +
#menu a {
 +
display: block;
 +
float: left;
 +
width: 95px;
 +
height: 45px;
 +
padding: 20px 0 0 0;
 +
border-left: 1px solid #EDEBD5;
 +
text-transform: lowercase;
 +
text-decoration: none;
 +
text-align: center;
 +
font-size: 135%;
 +
color: #BABABA;
 +
}
 +
 
 +
#menu a:hover {
 +
background: #F7F7F4;
 +
}
 +
 
 +
#menu .active a {
 +
background: #5dbb10;
 +
color: #FFFFFF;
 +
}
 +
/* Header */
 +
 
 +
#header {
 +
width: 770px;
 +
height: 170px;
 +
margin: 0 auto;
 +
}
 +
 
 +
#header h1 {
 +
float: left;
 +
width: 220px;
 +
height: 100px;
 +
margin: 0;
 +
padding: 70px 0 0 0;
 +
background: url(https://static.igem.org/mediawiki/2012/0/0a/Img02.jpg) no-repeat;
 +
text-align: center;
 +
font-size: 36px;
 +
}
 +
 
 +
#header h2 {
 +
float: right;
 +
width: 530px;
 +
height: 86px;
 +
margin: 0;
 +
background: url(https://static.igem.org/mediawiki/2012/4/4a/Img03.jpg) no-repeat;
 +
padding: 84px 20px 0 0;
 +
text-align: right;
 +
}
 +
 
 +
#header a {
 +
text-transform: lowercase;
 +
text-decoration: none;
 +
font-style: italic;
 +
font-weight: normal;
 +
color: #FFFFFF;
 +
}
 +
/* Page */
 +
 
 +
#page {
 +
width: 770px;
 +
        margin: 0 auto;
 +
padding: 20px 0;
 +
}
 +
 
 +
 
 +
/* Content */
 +
 
 +
#content {
 +
width: 760px;
 +
padding-top: 10px;
 +
}
 +
 
 +
.post {
 +
margin-bottom: 20px;
 +
padding: 0 20px;
 +
}
 +
 
 +
.hr {
 +
border-bottom: 1px solid #EDEBD5;
 +
}
 +
 
 +
.orangebox {
 +
background: url(https://static.igem.org/mediawiki/2012/b/b0/Img05.gif) repeat-y;
 +
}
 +
 
 +
.orangebox h2 {
 +
background: url(https://static.igem.org/mediawiki/2012/c/c2/Img06.gif) repeat-y;
 +
}
 +
/* Footer */
 +
 
 +
#footer {
 +
clear: both;
 +
height: 100px;
 +
padding: 25px 0;
 +
background: #EDEBD5 url(https://static.igem.org/mediawiki/2012/a/a5/Img04.gif) repeat-x;
 +
}
 +
 
 +
#footer p {
 +
margin: 0;
 +
text-align: center;
 +
font-size: 77%;
 +
}
 +
 
 +
#footer a {
 +
color: #666666;
 +
}
 +
#sponsors{
 +
        float: left;
 +
width: 88px;
 +
padding-top:15px;
 +
background: #EDEBD5;
 +
 +
      }
 +
 
 +
</style>
-
<!--Fireworks CS6 Dreamweaver CS6 target.  Created Tue Sep 25 17:05:18 GMT-0500 (Central Daylight Time (Mexico)) 2012-->
 
</head>
</head>
-
<body bgcolor="#ffffff">
+
<body>
-
<table style="display: inline-table;" border="0" cellpadding="0" cellspacing="0" width="950" bgcolor="">
+
<div id="menu">
-
<!-- fwtable fwsrc="INDEX.fw.png" fwpage="Page 1" fwbase="principal.jpg" fwstyle="Dreamweaver" fwdocid = "927795023" fwnested="0" -->
+
<ul>
-
  <tr>
+
<li><a href="home.html">Home</a></li>
-
  <td><img src="images/spacer.gif" width="240" height="1" alt="" /></td>
+
<li><a href="#">Rhodofactory</a></li>
-
  <td><img src="images/spacer.gif" width="710" height="1" alt="" /></td>
+
<li><a href="#">Promoter selection </a></li>
-
  <td><img src="images/spacer.gif" width="1" height="1" alt="" /></td>
+
<li><a href="#">results </a></li>
-
  </tr>
+
<li><a href="#">Perspectives</a></li>
-
<tr>
+
<li class="active"><a href="#">Modeling</a></li>
-
  <td><img src="https://static.igem.org/mediawiki/2012/6/68/LOGOPRIN.jpg" width="240" height="178" alt="Logo Principal" /></td>
+
<li><a href="aboutus.html">About us</a> </li>
-
  <td bgcolor="01A79D"><ul class="nav">
+
<li><a href="#">outreach</a></li>
-
<li>
+
</ul>
-
<a href="/Team/CINVESTAV-IPN-UNAM_MX/home.htm">Home<span class="flecha">&#9660;</span></a>
+
</div>
-
</li>
+
<!-- end #menu -->
-
<li>
+
<div id="header">
-
<a href="#">Rhodofactory<span class="flecha">&#9660;</span></a>
+
<h1><a href="#"></a></h1>  
-
<ul>
+
<h2><span></span></h2>
-
<li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Overview.htm">Overview<span class="flecha">&#9660;</span></a></li>
+
</div>
-
           
+
<!-- end #header -->
-
            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Light Response.htm">Light Response<span class="flecha">&#9660;</span></a></li>
+
<div id="page">
-
            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Oxigen Response.htm">Oxigen Response<span class="flecha">&#9660;</span></a></li>
+
<div id="content">
-
            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Chassis.htm">Chassis<span class="flecha">&#9660;</span></a></li>
+
  <div class="post hr">
-
            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Perspective.htm">Perspective<span class="flecha">&#9660;</span></a></li>
+
<h1><em>Rhodofactory</em></h1>
-
+
<h3>controlling genetic expression: an oxygen and light response </h3><br />
-
</ul>
+
<img src="https://static.igem.org/mediawiki/2012/c/ce/Imghome1.jpg" alt="" width="402" height="279" class="left" />
-
</li>
+
      <p>The metabolic versatility of purple non-sulfur photosynthetic bacteria  allows them to grow in light, darkness and with or without oxygen; all  it is due to their genetic regulation mechanisms. Taking advantage of  this, our project aims to build two genetic control systems based on <em>R. sphaeroides</em> photosynthesis cluster regulation. </p>
-
<li>
+
  <p>The first one is a light dependent system controlled by two proteins AppA/PpsR that works like an antirepresor/repressor mechanism, and the second one is an oxygen dependent system of two-component called PrrA/PrrB. This two devices were tested on R. palustris chassis, using a cassete in which a reporter (GFP) is regulated by external conditions that activate or repress its expresion. </p>
-
<a href="">Results<span class="flecha">&#9660;</span></a>
+
  <p>Once we have characterized the functionality of these networks, our per;spective is to develop a Rhodofactory, it means to control the produccion of differents metabolites, such as biodiesel and butanol, using simple signals.   </p>
-
<ul>
+
  <p>&nbsp;</p>
-
<li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Biobricks.htm">Biobricks<span class="flecha">&#9660;</span></a></li>
+
  </div>
-
           
+
  <div class="post"></div>
-
            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Notebook.htm">Notebook<span class="flecha">&#9660;</span></a></li>
+
-
            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Protocol.htm">Protocol<span class="flecha">&#9660;</span></a></li>
+
-
            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/palustris response.htm">R. palustris response<span class="flecha">&#9660;</span></a></li>
+
-
            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/sphaeroides response.htm">R. sphaeroides response<span class="flecha">&#9660;</span></a></li>
+
-
</ul>
+
-
</li>
+
-
+
-
<li>
+
-
<a href="#">IGEM Contribution<span class="flecha">&#9660;</span></a>
+
-
<ul>
+
-
<li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Construction.htm">Construction<span class="flecha">&#9660;</span></a></li>
+
-
           
+
-
            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Metods.htm">Methods<span class="flecha">&#9660;</span></a></li>
+
-
            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Results.htm">Results<span class="flecha">&#9660;</span></a></li>
+
-
+
-
</ul>
+
-
</li>
+
-
<li>
+
-
<a href="/Team/CINVESTAV-IPN-UNAM_MX/Modelling.htm">Modeling<span class="flecha">&#9660;</span></a>
+
-
+
-
</li>  
+
-
   
+
-
    <li>
+
-
<a href="#">Team<span class="flecha">&#9660;</span></a>
+
-
<ul>
+
-
<li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Members.htm">Members<span class="flecha">&#9660;</span></a></li>
+
-
           
+
-
            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Institute.htm">Institute<span class="flecha">&#9660;</span></a></li>
+
-
            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Labs.htm">Labs<span class="flecha">&#9660;</span></a></li>
+
-
            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Sponsor.htm">Sponsor<span class="flecha">&#9660;</span></a></li>
+
-
            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Gallery.htm">Gallery<span class="flecha">&#9660;</span></a></li>
+
-
            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Acknowledgments.htm">Acknowledgments<span class="flecha">&#9660;</span></a></li>
+
-
+
-
</ul>
+
-
</li>
+
-
    <li>
+
-
<a href="#">Outreach<span class="flecha">&#9660;</span></a>
+
-
<ul>
+
-
            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Human Practice.htm">Human Practices<span class="flecha">&#9660;</span></a></li>
+
-
            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Safety.htm">Safety<span class="flecha">&#9660;</span></a></li>
+
-
</ul>
+
-
</li>
+
-
   
+
-
   
+
-
</ul></td>
+
-
  <td><img src="images/spacer.gif" width="1" height="150" alt="" /></td>
+
-
  </tr>
+
-
  <tr>
+
-
  <td colspan="2" >
+
-
 
+
-
  <div id="main">
+
-
<div id="bodys_r1_c2">
+
</div>
</div>
-
<div id="bodys_r1_c3">
+
  <!-- end #content -->
-
   
+
  <!-- end #image2 -->
-
      <img src="https://dl.dropbox.com/u/199422/Cinvestav/images/Home%201.fw.png" width="500" height="92" /> </div>
+
      </p>
-
<div class="clearFloat"></div>
+
<div style="clear: both;">&nbsp;</div>
-
<div id="bodys_r2_c2">
+
</div>
-
   
+
<!-- end #page -->
-
      <img src="https://static.igem.org/mediawiki/2012/f/f3/RHODOFACTORY_rojo-09.fw.png" width="199" height="279" /> </div>
+
<div id="footer">
-
<div id="bodys_r2_c3">
+
<p id="legal"> Rhodofactory 2012 </p>
-
    <div class="Text">
+
<div id="sponsors">
-
   
+
  <div align="center"><img src="https://static.igem.org/mediawiki/2012/8/8a/Icytdf.png" alt="icytdf" width="70" height="99" /></div>
-
      <p>The metabolic versatility of purple non-sulfur photosynthetic bacteria allows them to grow in light, darkness and with or without oxygen; all it is due to their genetic regulation mechanisms. Taking advantage of this, our project aims to build two genetic control systems based on <em>R. sphaeroides</em> photosynthesis regulation. </p>
+
</div>
-
      <p>The first one is a light dependent system controlled by two proteins that works as an antirepresor/repressor mechanism, and the second one is an oxygen dependent two-component system. We tested these devices on <em>R. palustris</em>. We built a genetic circuit that activate or repress GFP expression in response to external conditions.</p>
+
<div id="sponsors">
-
       <p> Once we characterize the functionality of these networks, we want to create a Rhodofactory to use our systems to control production butanol using simple signals. </p>
+
  <div align="center"><img src="https://static.igem.org/mediawiki/2012/6/67/Osli.png" alt="osli" width="88" height="46" /></div>
-
    </div>
+
</div>
 +
 +
<div id="sponsors">
 +
  <div align="center"><img src="https://static.igem.org/mediawiki/2012/f/fb/Bio.png" alt="bio" width="73" height="99" /></div>
 +
</div>
 +
<div id="sponsors"><img src="https://static.igem.org/mediawiki/2012/d/d6/Fermentas.png" alt="fermentAS" width="88" height="51" /></div>
 +
<div id="sponsors">
 +
  <div align="center"><img src="https://static.igem.org/mediawiki/2012/a/a3/Cinestav.png" alt="cinestav" width="76" height="99" /></div>
 +
</div>
 +
<div id="sponsors"><img src="https://static.igem.org/mediawiki/2012/9/9b/Genscript.png" alt="genscript" width="88" height="51" /></div>
 +
<div id="sponsors">
 +
  <div align="center"><img src="https://static.igem.org/mediawiki/2012/1/16/Unam.png" alt="unam" width="82" height="99" /></div>
 +
</div>
 +
<div id="sponsors"><img src="https://static.igem.org/mediawiki/2012/c/c9/Gto.png" alt="gto" width="88" height="53" /></div>
 +
    <div id="sponsors">
 +
       <div align="center"><img src="https://static.igem.org/mediawiki/2012/f/fa/Qimica.png" alt="quimica" width="78" height="90" /></div>
     </div>
     </div>
-
<div class="clearFloat"></div>
+
<div id="sponsors"><img src="https://static.igem.org/mediawiki/2012/7/73/Valaner.png" alt="valaner" width="88" height="55" /></div>
 +
<div id="sponsors">
 +
  <div align="center"><img src="https://static.igem.org/mediawiki/2012/7/79/Ipn.png" alt="ipn" width="75" height="97" /></div>
 +
</div>
 +
</div>
</div>
-
 
+
<!-- end #footer -->
-
    </td>
+
-
  <td><img src="https://dl.dropbox.com/u/199422/Cinvestav/images/spacer.gif" width="1" height="500" alt="" /></td>
+
-
  </tr>
+
-
</table>
+
</body>
</body>
</html>
</html>

Revision as of 03:01, 22 October 2012

Rho

Rhodofactory

controlling genetic expression: an oxygen and light response


The metabolic versatility of purple non-sulfur photosynthetic bacteria allows them to grow in light, darkness and with or without oxygen; all it is due to their genetic regulation mechanisms. Taking advantage of this, our project aims to build two genetic control systems based on R. sphaeroides photosynthesis cluster regulation.

The first one is a light dependent system controlled by two proteins AppA/PpsR that works like an antirepresor/repressor mechanism, and the second one is an oxygen dependent system of two-component called PrrA/PrrB. This two devices were tested on R. palustris chassis, using a cassete in which a reporter (GFP) is regulated by external conditions that activate or repress its expresion.

Once we have characterized the functionality of these networks, our per;spective is to develop a Rhodofactory, it means to control the produccion of differents metabolites, such as biodiesel and butanol, using simple signals.

 

 

Retrieved from "http://2012.igem.org/Team/CINVESTAV-IPN-UNAM_MX/home.htm"