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Team:Freiburg
From 2012.igem.org
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'''Let us tell you a fabulous TALE ...'''<br> | '''Let us tell you a fabulous TALE ...'''<br> | ||
| - | Transactivator-Like Effectors (TALEs) are a brand-new technology that currently revolutionizes the way researchers manipulate DNA with exceptional site specificity. Originally derived from Xanthomonas spp., this type of protein comprizes an effector domain and a modular DNA binding domain that can be rationally designed to bind to virtually any target sequence of DNA. Over the past two years, universal endonucleases (TALENs) and transcription factors have been tested in various organisms ranging from bacteria to humans. According to existing protocols, TALE assembly requires several weeks of work and substantial lab skills. In order to bring this technology within reach for iGEM students, we invented an extremely fast and easy TALE assembly strategy and developed a TALE platform with expression plasmids and new classes of TALEs. With our so called GATE assembly kit, future iGEM students will be able to precisely manipulate genomic loci easier and faster than anyone else in the world. | + | Transactivator-Like Effectors (TALEs) are a brand-new technology that currently revolutionizes the way researchers manipulate DNA with exceptional site specificity. Originally derived from ''Xanthomonas spp.'', this type of protein comprizes an effector domain and a modular DNA binding domain that can be rationally designed to bind to virtually any target sequence of DNA. Over the past two years, universal endonucleases (TALENs) and transcription factors have been tested in various organisms ranging from bacteria to humans. According to existing protocols, TALE assembly requires several weeks of work and substantial lab skills. In order to bring this technology within reach for iGEM students, we invented an extremely fast and easy TALE assembly strategy and developed a TALE platform with expression plasmids and new classes of TALEs. With our so called GATE assembly kit, future iGEM students will be able to precisely manipulate genomic loci easier and faster than anyone else in the world. |
Revision as of 16:27, 9 October 2012
on this wiki, we want to present our project for the iGEM 2012 competition and introduce the freiGEM 2012 team.
Let us tell you a fabulous TALE ...
Transactivator-Like Effectors (TALEs) are a brand-new technology that currently revolutionizes the way researchers manipulate DNA with exceptional site specificity. Originally derived from Xanthomonas spp., this type of protein comprizes an effector domain and a modular DNA binding domain that can be rationally designed to bind to virtually any target sequence of DNA. Over the past two years, universal endonucleases (TALENs) and transcription factors have been tested in various organisms ranging from bacteria to humans. According to existing protocols, TALE assembly requires several weeks of work and substantial lab skills. In order to bring this technology within reach for iGEM students, we invented an extremely fast and easy TALE assembly strategy and developed a TALE platform with expression plasmids and new classes of TALEs. With our so called GATE assembly kit, future iGEM students will be able to precisely manipulate genomic loci easier and faster than anyone else in the world.
Stay tuned to FreiGEM 2012 - we will keep you in the loop.
Why we think we deserve a gold medal in the iGEM 2012 contest:
