Team:Virginia/Parts

From 2012.igem.org

(Difference between revisions)

Revision as of 03:21, 4 October 2012

1. Human Chorionic Gonadotropin, Beta Subunit (hCG-β) - BBa_K732000
Human chorionic gonadotropin (hCG) is a hormone produced during pregnancy. Its beta subunit (hCG-β) contains epitopes recognized by pregnancy tests. The full hCG dimer is also used as an ovulation inducer and can stimulate the production of testosterone in men. This sequence can be used to express hCG for any downstream uses, and also as an output signal since it can trigger pregnancy tests at low concentrations.

2. Synthetic Chorionic Gonadotropin, beta (synCG-β) - BBa_K732001
A synthetic version of human chorionic gonadotropin (hCG) codon-optimized for bacterial expression and sequence composability. For ease of synthesis, synonymous codons of slightly lower usage bias were used in some cases to reduce GC content and avoid hairpin structures.

3. synCG-β + Promoter + RBS - BBa_K732002
This construct contains a strong constitutive promoter (J23119) as well as an RBS (B0034) upstream of synCG-β (K732001) to strongly express a synthetic version of the beta subunit of human chorionic gonadotropin.

4. bbp9 BBa_K732003
Putative lysin from the Bordetella phage genome. Contains sequence similarity to ElmA, a protein in E. coli that breaks down capsid polysaccharides.
iGEM012 Virginia

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+<b>1. Human Chorionic Gonadotropin, Beta Subunit (hCG-β) - <a href="http://partsregistry.org/wiki/index.php/Part:BBa_K732000">BBa_K732000</a></b><br>
+Human chorionic gonadotropin (hCG) is a hormone produced during pregnancy. Its beta subunit (hCG-β) contains epitopes recognized by pregnancy tests. The full hCG dimer is also used as an ovulation inducer and can stimulate the production of testosterone in men. This sequence can be used to express hCG for any downstream uses, and also as an output signal since it can trigger pregnancy tests at low concentrations.<br>
+<br>
-An important aspect of the iGEM competition is the use and creation of standard  biological parts. Each team will make new parts during iGEM and will place them in the [http://partsregistry.org Registry of Standard Biological Parts]. The iGEM software provides an easy way to present the parts your team has created . The "groupparts" tag will generate a table with all of the parts that your team adds to your team sandbox.  Note that if you want to document a part you need to document it on the [http://partsregistry.org Registry], not on your team wiki.
+<b>2. Synthetic Chorionic Gonadotropin, beta (synCG-β) - <a href="http://partsregistry.org/wiki/index.php/Part:BBa_K732001">BBa_K732001</a> <br></b>
+A synthetic version of human chorionic gonadotropin (hCG) codon-optimized for bacterial expression and sequence composability. For ease of synthesis, synonymous codons of slightly lower usage bias were used in some cases to reduce GC content and avoid hairpin structures.<br>
+<br>
-Remember that the goal of proper part documentation is to describe and define a part such that it can be used without a need to refer to the primary literature. The next iGEM team should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for  users who wish to know more.
+<b>3. synCG-β + Promoter + RBS - <a href="http://partsregistry.org/wiki/index.php/Part:BBa_K732002">BBa_K732002</a><br></b>
+This construct contains a strong constitutive promoter (J23119) as well as an RBS (B0034) upstream of synCG-β (K732001) to strongly express a synthetic version of the beta subunit of human chorionic gonadotropin.<br>
+<br>
+<b>4. bbp9 <a href="http://partsregistry.org/wiki/index.php/Part:BBa_K732003">BBa_K732003</a><br></b>
+Putative lysin from the Bordetella phage genome. Contains sequence similarity to ElmA, a protein in E. coli that breaks down capsid polysaccharides. <br>
 <groupparts>iGEM012 Virginia</groupparts>