Team:USC/Project2

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Results

E. coli Environment Alters Tumbling:

#3transition text here

Salt Experiment

CONTROL

0.0 M NaCl

RPS (rotations/sec)	0.21	0.17	0.48	0.71	0.45
Frequency (Hz)	132	107	302	446	283

0.5 M NaCl

RPS (rotations/sec)	0.80	0.83	0.72	0.71	0.71
Frequency (Hz)	503	522	452	446	446

0.9 M NaCl

RPS (rotations/sec)	0.21	0.55	0.71	0.40	0.54
Frequency (Hz)	132	346	446	251	339

CheY

0.0 M NaCl

RPS (rotations/sec)	0.57	0.60	0.68	0.78	0.74
Frequency (Hz)	358	377	427	490	465

0.5 M NaCl

RPS (rotations/sec)	0.32	0.40	0.43	0.25	0.35
Frequency (Hz)	201	251	270	157	220

0.9 M NaCl

RPS (rotations/sec)	0.31	0.32	0.21	0.25	0.28
Frequency (Hz)	195	201	132	157	176

CheZ

0.9 M NaCl

RPS (rotations/sec)	0.63	0.50	0.25	0.63	0.31
Frequency (Hz)	396	314	157	395	195

CheY vs. CONTROL

Salt Concentration	0.0M NaCl		0.5M NaCl		0.9M Nacl
	Control	CheY	Control	CheY	Control	CheY
Avgerage frequency (Hz)	254	424	474	220	303	172
Standard Deviation	138	56	36	44	118	28
Two-tailed t-test (a=0.05)	0.0127		0.0003		0.0978

CheY vs. CheZ vs. CONTROL

	Control	cheY	cheZ
Avgerage frequency (Hz)	303	172	292
Standard Deviation	28	118	115
Two-tailed t-test (a=0.05)		0.0978	0.9136

#4Transition text here

Inducible Tumbling System in cheY and cheZ

In order to test our bacterial communication system, we transformed cheY and cheZ parts into E. coli B strain HT115. Our BioBricks included an inducible T7 promoter that is activated by IPTG. As shown in the figure, 100% of successfully transformed bacteria with the T7 RBS cheY construct showed tumbling, demonstrating the functionality of the T7 RBS cheY part.

Reconstitution of Flagella

Our team created the following BioBricks that control the assembly and operon regulation of E. coli flagella: BBa_K842000 flhB, BBa_K842001 flhD, BBa_K842002 flgJ, BBa_842003 fliA, BBa_K842005 fliH, BBa_K842011 motA, BBa_K842012 motB. These parts allow for the reconstitution of a flagella mechanism in E. coli B strains where the flagella mechanisms are lacking.

Future Directions

#5transition text here

pH Experiment

CONTROL

pH 5

RPS (rotations/sec)	0.25	0.33	0.36	0.20	0.42
Frequency (Hz)	157	207	226	126	264

pH 6

RPS (rotations/sec)	0.43	0.36	0.46	0.40	0.25
Frequency (Hz)	270	226	289	251	157

pH 7

RPS (rotations/sec)	0.21	0.17	0.48	0.71	0.45
Frequency (Hz)	132	107	302	446	283

pH 8

RPS (rotations/sec)	0.25	0.50	0.25	0.35	0.33
Frequency (Hz)	157	314	157	220	207

pH 9

RPS (rotations/sec)	0.26	0.25	0.33	0.33	0.32
Frequency (Hz)	163	157	207	207	201

pH	5	6	7	8	9
Average frequency (Hz)	196	238	254	211	187
Standard Deviation	55	51	138	64	24

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 						<p><span style="color:#000000;"><strong><em><span style="text-decoration:underline;">E. coli </span></em><span style="text-decoration:underline;">Environment Alters Tumbling:</span></strong></span></p>
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+<p><span style="color:#000000;">#3transition text here</span></p>
 <p><span style="color:#000000;"><strong><span style="text-decoration:underline;">Salt Experiment</span></strong></span></p>
 <p><span style="color:#000000;"><strong><span style="text-decoration:underline;">CONTROL</span></strong></span></p>
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 </table>
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 <p><span style="color:#000000;"><strong><span style="text-decoration:underline;">Inducible Tumbling System in cheY and cheZ</span></strong></span></p>
 <p><span style="color:#000000;">In order to test our bacterial communication system, we transformed cheY and cheZ parts into <em>E. coli</em> B strain HT115. Our BioBricks included an inducible T7 promoter that is activated by IPTG. As shown in the figure, 100% of successfully transformed bacteria with the T7 RBS cheY construct showed tumbling, demonstrating the functionality of the T7 RBS cheY part.</span></p>
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 <p><span style="color:#000000;">Our team created the following BioBricks that control the assembly and operon regulation of <em>E. coli</em> flagella: BBa_K842000 flhB, BBa_K842001 flhD, BBa_K842002 flgJ, BBa_842003 fliA, BBa_K842005 fliH, BBa_K842011 motA, BBa_K842012 motB. These parts allow for the reconstitution of a flagella mechanism in E. coli B strains where the flagella mechanisms are lacking.</span></p>
 <p><span style="color:#000000;"><strong><span style="text-decoration:underline;">Future Directions</span></strong></span></p>
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+<p><span style="color:#000000;">#5transition text here</span></p>
 <p><span style="color:#000000;"><strong><span style="text-decoration:underline;">pH Experiment</span></strong></span></p>
 <p><span style="color:#000000;"><strong><span style="text-decoration:underline;">CONTROL</span></strong></span></p>