Team:Lethbridge/notebook

From 2012.igem.org

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<h2 class="pagetitle">June 2012</h2>
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<p>Content Here</p>
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<p>Read More...</p>
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<p>Procedure:</p>
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<p>Extraction of DNA from kit plates</p>
<p>Extraction of DNA from kit plates</p>
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<li>10 µL of Milli-Q H2O and extract parts from the kit plates
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Transformation into E. coli DH5</li>
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<li>Add 2 µL of DNA extract to 20 µL of E. coli DH5</li>
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<li>Incubate on ice for 30 min; 30 sec in water bath at 42˚C</li>
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<li>Immediately on ice for 5 min</li>
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<li>Add 400 µL of SOC media & resuspend</li>
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<li>Incubate for 1 hr at 37˚C in shaker</li>
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<li>Plate 200 µL of transformed E. coli on correct antibiotic plate</li>
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<li>Leave overnight in incubator at 37˚C</li>
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<p><a href="http://reamsbottom.net/igem/docs/JulyKillSwitchNotebook.pdf">Read More Entries from July by clicking here...</a></p>  
<p><a href="http://reamsbottom.net/igem/docs/JulyKillSwitchNotebook.pdf">Read More Entries from July by clicking here...</a></p>  
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<h2>August and September 2012</h2>
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<h2>August 2012</h2>
<p>Content Here</p>  
<p>Content Here</p>  
<p><a href="http://reamsbottom.net/igem/docs/July30-SeptemberKillswitchNotebook.pdf">Read More Entries from August by clicking here...</a></p>  
<p><a href="http://reamsbottom.net/igem/docs/July30-SeptemberKillswitchNotebook.pdf">Read More Entries from August by clicking here...</a></p>  

Revision as of 02:32, 4 October 2012

2012 iGEM - University of Lethbridge

Notebook

Our notebooks document the University of Lethbridge's iGEM Team through their scientific journey

July 2012

Date: July 13th, 2012

Experimenters: Jenn & Boris

Transformation of Multiple Kill Switches

Objective: To extract DNA from kit plates and transform into E. coli DH5

  • K112806 from 2012 kit plate 4 well 6M
  • K112808 from 2012 kit plate 3 well 6G
  • K117000 from 2012 kit plate 2 well 14D
  • K124014 from 2012 kit plate 3 well 20K

Procedure:

Extraction of DNA from kit plates

Read More Entries from July by clicking here...

August 2012

Content Here

Read More Entries from August by clicking here...

September 2012

Content Here

Read More...

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