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Team:HokkaidoU Japan/Notebook
From 2012.igem.org
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| Line 101: | Line 101: | ||
*7th | *7th | ||
'''3A assembly!''' | '''3A assembly!''' | ||
| + | Assembled pT7, RBS and pSB1C3 by 3A assembly. | ||
| + | This 3A assembly is our first try! | ||
| + | |||
;mini-prep | ;mini-prep | ||
#mini-prep of dT,RBS,pT7 and pLacI-RBS-Ag43. | #mini-prep of dT,RBS,pT7 and pLacI-RBS-Ag43. | ||
| + | #Elution in 50ul buffer | ||
<br> | <br> | ||
| Line 109: | Line 113: | ||
#Parts written above were cultivated in LBA(dT,RBS,pT7) and LBC(pLacI-RBS-Ag43) 1ml in 16hrs 30min. | #Parts written above were cultivated in LBA(dT,RBS,pT7) and LBC(pLacI-RBS-Ag43) 1ml in 16hrs 30min. | ||
#Add glycerol and Freeze at -80C | #Add glycerol and Freeze at -80C | ||
| + | |||
| + | ;Electrophoresis | ||
| + | Electrophoresis to predict concentration of mini-prep products(dT,RBS,pT7 and pLacI-RBS-Ag43). | ||
| + | #Used 1% agarose gel. | ||
| + | #Pre-migration. | ||
| + | #Migrated 1.2ul of DNA solutions (1ul is mini-prep products and 0.2ul is Loading Dye) in OOmin. | ||
| + | |||
| + | ;Digestion | ||
| + | ;Ligation | ||
| + | ;Transformation | ||
Revision as of 04:57, 7 July 2012
| Home | Team | Official Team Profile | Project | Parts Submitted to the Registry | Modeling | Notebook | Safety | Attributions |
|---|
Contents |
Hello
We are team HokkaidoU Japan! Today we learn and start to edit wiki.
(>ω<)
Dear Mr.Ortiz, I saw the help page which you edited.
hola!
March
Spring Boot Camp
- date
- March 5 (Mon) ~ March 9 (Fri)
Monday, March 5
- Session #1
- Short lecture about moleculer biology (Mr.Yamazaki, our adviser)
- Session #2
- Tutrial: How to use 'Unipro UGENE' (iTakeshi)
- Session #3
- Guidance: Wiki Reading (Laury)
- Example: 2010 MIT
Tuesday, March 6
- Session #4~6
- Reading Wikis in turn and discussions
- 2010 NYU
- 2009 Cambridge
- 2009 Growningen
Wednesday, March 7
- Session #7~11
- Reading Wikis (2)
- 2010 Washington
- 2009 Valencia
- 2011 Barklay
- 2010 Paris
- 2010 Bristol
Thursday, March 8
- Session #12
- 2012 Project Brainstorming
- The details is secret! :)
- Session #13
- Guidance: How to read papers (Laury)
Friday, March 9
- Session #14
- 2012 Project Brainstorming (2)
- Session #15
- Guidance: How to look up papers you want (Laury)
- Session #16
- Tutorial: Modeling the behavior of cells (iTakeshi)
- Session #17
- Final Session: Reviewing this camp
- Party!!
July
phaABC team
now experimenting...
Ag43&Lysis team experiment
weak 1
- 4th
- Transformation
- Transformation of BBa_B0015(dT), B0034(RBS), I179005(pT7), K346007(Ag43), and K542009(pLacI-RBS-Ag43) in DH5α
- Cultivated on LBA(dt,RBS,T7) and LBC(Ag43, pLacI-RBS-Ag43) . pT7 was 21hrs and Others were 20hrs cultivated
- 5th
- Transformation
K346007(Ag43) was failed to cultivate on LBC plate.
- Transformation of K346007(Ag43) in DH5α.
- Cultivated on LBC in 21hrs.
- Single colony isolation
- Single colony isolation of BBa_B0015, B0034, I179005 and K542009.
- Cultivation on LBA(dt,RBS,T7) and LBC(pLacI-RBS-Ag43) in 14hrs30mins
BBa_K542009 was Ag43 only part! And the part didn't have Biobrick suffix.
- 6th
- Liquid culture
- Liquid culture in LBA(dT,RBS,pT7) and LBC(pLacI-RBS-Ag43)
- LB 1ml is for glycerol stocks and 2ml is for mini-prep.
- 16hrs Cultivation
- Single colony isolation
- Single colony isolation of K346007(Ag43).
- 7th
3A assembly! Assembled pT7, RBS and pSB1C3 by 3A assembly. This 3A assembly is our first try!
- mini-prep
- mini-prep of dT,RBS,pT7 and pLacI-RBS-Ag43.
- Elution in 50ul buffer
- Glycerol stock
Made glycerol stocks of dT,RBS,pT7 and pLacI-RBS-Ag43.
- Parts written above were cultivated in LBA(dT,RBS,pT7) and LBC(pLacI-RBS-Ag43) 1ml in 16hrs 30min.
- Add glycerol and Freeze at -80C
- Electrophoresis
Electrophoresis to predict concentration of mini-prep products(dT,RBS,pT7 and pLacI-RBS-Ag43).
- Used 1% agarose gel.
- Pre-migration.
- Migrated 1.2ul of DNA solutions (1ul is mini-prep products and 0.2ul is Loading Dye) in OOmin.
- Digestion
- Ligation
- Transformation
