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Team:Washington/Protocols/Digestion
From 2012.igem.org
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If two enzymes are required, check http://www.neb.com/nebecomm/DoubleDigestCalculator.asp for buffer, temperature, BSA specifications. | If two enzymes are required, check http://www.neb.com/nebecomm/DoubleDigestCalculator.asp for buffer, temperature, BSA specifications. | ||
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Latest revision as of 02:14, 4 October 2012
General Digestion Protocol
10 uL DNA
5uL buffer (check http://www.neb.com/nebecomm/EnzymeFinderSearchByName.asp for a list of enzymes and associated buffers)
0.5 uL BSA (if required. See above)
1uL enzyme 1
water to 50 uL (32.5 uL, add first)
If two enzymes are required, check http://www.neb.com/nebecomm/DoubleDigestCalculator.asp for buffer, temperature, BSA specifications.
