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Team:Lethbridge/notebook
From 2012.igem.org
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<h2>July 2012</h2> | <h2>July 2012</h2> | ||
| - | <p> | + | <p>Date: July 13th, 2012</p> |
| - | <p>Read More...</p> | + | <p>Experimenters: Jenn & Boris</p> |
| + | <p>Transformation of Multiple Kill Switches</p> | ||
| + | <p>Objective: To extract DNA from kit plates and transform into E. coli DH5</p> | ||
| + | <p> | ||
| + | <ul> | ||
| + | <li>K112806 from 2012 kit plate 4 well 6M </li> | ||
| + | <li>K112808 from 2012 kit plate 3 well 6G </li> | ||
| + | <li>K117000 from 2012 kit plate 2 well 14D </li> | ||
| + | <li>K124014 from 2012 kit plate 3 well 20K </li> | ||
| + | </ul> | ||
| + | </p> | ||
| + | <p>Procedure:</p> | ||
| + | <p>Extraction of DNA from kit plates</p> | ||
| + | <p> | ||
| + | <ul> | ||
| + | <li>10 µL of Milli-Q H2O and extract parts from the kit plates | ||
| + | Transformation into E. coli DH5</li> | ||
| + | <li>Add 2 µL of DNA extract to 20 µL of E. coli DH5</li> | ||
| + | <li>Incubate on ice for 30 min; 30 sec in water bath at 42˚C</li> | ||
| + | <li>Immediately on ice for 5 min</li> | ||
| + | <li>Add 400 µL of SOC media & resuspend</li> | ||
| + | <li>Incubate for 1 hr at 37˚C in shaker</li> | ||
| + | <li>Plate 200 µL of transformed E. coli on correct antibiotic plate</li> | ||
| + | <li>Leave overnight in incubator at 37˚C</li> | ||
| + | </ul> | ||
| + | </p> | ||
| + | <p><a href="http://reamsbottom.net/igem/docs/JulyKillSwitchNotebook.pdf">Read More Entries from July by clicking here...</a></p> | ||
</div> | </div> | ||
Revision as of 01:01, 4 October 2012
Notebook
Our notebooks document the University of Lethbridge's iGEM Team through their scientific journey
June 2012
Content Here
Read More...
July 2012
Date: July 13th, 2012
Experimenters: Jenn & Boris
Transformation of Multiple Kill Switches
Objective: To extract DNA from kit plates and transform into E. coli DH5
- K112806 from 2012 kit plate 4 well 6M
- K112808 from 2012 kit plate 3 well 6G
- K117000 from 2012 kit plate 2 well 14D
- K124014 from 2012 kit plate 3 well 20K
Procedure:
Extraction of DNA from kit plates
- 10 µL of Milli-Q H2O and extract parts from the kit plates Transformation into E. coli DH5
- Add 2 µL of DNA extract to 20 µL of E. coli DH5
- Incubate on ice for 30 min; 30 sec in water bath at 42˚C
- Immediately on ice for 5 min
- Add 400 µL of SOC media & resuspend
- Incubate for 1 hr at 37˚C in shaker
- Plate 200 µL of transformed E. coli on correct antibiotic plate
- Leave overnight in incubator at 37˚C
August 2012
Content Here
Read More...
September 2012
Content Here
Read More...
