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Team:IvyTech-South Bend/Notebook
From 2012.igem.org
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-Pulled K190015 because its an arsenic sensitive promoter ẅ RFP | -Pulled K190015 because its an arsenic sensitive promoter ẅ RFP | ||
| - | - | + | -Transformed K190015 on Psb1A13 |
| + | |||
| + | July 16, 2012 Week 5. | ||
| + | Propped Test promoter and transferred to Arsenic inducible promoter J33201 with Ars R repressor binding site. To be able to test for decreasing sensitivity to Arsenic. | ||
Revision as of 00:10, 4 October 2012
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<a href="#">Lab Notebook: See our weekly lab work</a>
June 18, 2012 Week 1. Staging and presentation of project storming to Ivy Tech advisory board. Parts research and descriptions.
June 25, 2012 Week 2. Continuation of parts research including promoters & killswitch screening and selction. Constitutive PTeT promoter, RBS, double terminator.
July 2, 2012 Week 3. Checked cultures and paired plates. Continuation of parts research and construction
July 9, 2012 Week 4. Oops Event!!! Protocol correction due to low yield or transformation. Killswitch transformation was redone (serial dilution test for transformation efficiency) Kamamiosin proves to be better selection for transformation for the psb1Ak3 plasmid backbone.
-Pulled K190015 because its an arsenic sensitive promoter ẅ RFP
-Transformed K190015 on Psb1A13
July 16, 2012 Week 5. Propped Test promoter and transferred to Arsenic inducible promoter J33201 with Ars R repressor binding site. To be able to test for decreasing sensitivity to Arsenic.
