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          <h6 class="clr-9 p8">NoteBook</h6>
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            <h6 class="clr-9 p8">Safety</h6>
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   <p class="text-3 p3"></p>
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   <p class="text-3 p3">1. Would any of your project ideas raise safety issues in terms of researcher, public, or environmental safety?</p>
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             <p>The organisms used in our lab are well documented and generally regarded as safe (GRAS). These organisms are nonpathogenic and are not harmful for use in the lab. The GSU iGEM team follows lab safety very carefully. Lab goggles, long coats, gloves, and proper attire is always required while working in the lab. Additionally, all recombinant organisms were disposed of properly, ensuring no accidental release into the environment. Our project ideas did not raise any other safety issues. .</p>
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            <div class="date">27<span>May</span></div>
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            <p class="text-3 top-1 p3">2. Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? If yes, did you document these issues in the Registry? How did you manage to handle the safety issue? How could other teams learn from your experience?</p>
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                <div class="extra-wrap">
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             <p class="p1">Activities in the development of BioBrick parts did not pose an issue to the GSU iGEM team. We created out own linker piece to fit the iGEM standard with no issues. This linker piece can be used to make most all commercially-available vectors fit the iGEM assembly standard. We fully comply with the National Institutes of Health Guidelines for Recombinant DNA. </p>
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                <p><a href="#" class="link-1">Week 1.</a></p>
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            <p class="text-3 top-1 p3">3. Is there a local biosafety group, committee, or review board at your institution? If yes, what does your local biosafety group think about your project? If no, which specific biosafety rules or guidelines do you have to consider in your country?</p>
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                    Research (both pichia and agrobacterium)- Merhawi, Latara, Khamani, Morgan, Alyssa, Payal, and Mary
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             <p class="p1">GSU has an Institutional Biosafety Committee. The team has complied with all safety rules and regulations throughout the project. All routine lab inspections were conducted by the Biosafety Officer, and all members have been properly trained on autoclaving, dishwashing, and disposal of biohazardous and chemical wastes. The link for the GSU Biosafety Website is: <a href="http://www.gsu.edu/research/ibc.html">http://www.gsu.edu/research/ibc.html</a></p>
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            <p class="text-3 top-1 p3">4. Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices, and systems be made even safer through biosafety engineering?</p>
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                <div class="clear"></div>
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             <p class="p1">In the future, iGEM could provide teams with mini, online courses to give an overview/introduction of biosafety regulations and guidelines. The courses could be followed by a small quiz that all participating teams must pass. This would ensure that students have a better understanding of safety in the lab. In conjunction with GSU, all members working in a laboratory must pass the CITI online training. </p>
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            <div class="date">03<span>jun</span></div>
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                <p><a href="#" class="link-1">Week 2.</a></p>
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                    Made YEB medium- Merhawi. Sequencing Plasmids- Alyssa, Payal, and Latara.
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Research- Khamani
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Finish pgap gene test- Khamani. Had a group meeting!
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            <div class="date">10<span>jun</span></div>
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                <p><a href="#" class="link-1">week 3.</a></p>
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                    DNA Transformation - Merhawi and Dafna Made YEB plates- Khamani, Payal, and Mary Made LB agar plates with Kanamycin - Alyssa and Latara Planting - Dafna and Merhawi
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            <div class="date">17<span>jun</span></div>
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                <p><a href="#" class="link-1">week 4.</a></p>
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                    Mini prep - Merhawi, Alyssa and Dafna Made SOC medium Merhawi Made some YPD medium- Khamani Preparing cells for transformation (pichia)- Mary Ti Transformation-Innoculated large culture for midi prep, watered plants and electroporation of cells - Latara
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            <div class="date">24<span>jun</span></div>
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                <div class="extra-wrap">
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                <p><a href="#" class="link-1">week 5.</a></p>
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                    Grew some E. Coli cells- Khamani Culture Ti plasmids, plating and mini plasmid extraction - Alyssa and Merhawi Midi prep - Payal, Khamani, and Mary Gel electrophoresis and gel pictures - Merhawi and Alyssa Restriction digest and made agarose geland PCR- Khamani, Payal, and Mary
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            <div class="date">01<span>jul</span></div>
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                <div class="extra-wrap">
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                <p><a href="#" class="link-1">week 6.</a></p>
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                    PCR rd gel?- Khamani Restriction digest - Payal and Mary PCR, Rd gel - Merhawi and Dafna Found DNA concentration of?- Khamani, Payal, and Mary DNA gel extraction- Khamani Quantification of 20I DNA - Latara Transformation, RFP mini prep, agrose gel, and restriction digest of pORE expression series - Alyssa
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            <div class="date">30<span>jun</span></div>
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                <div class="extra-wrap">
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                <p><a href="#" class="link-1">week 7.</a></p>
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                    Mini prep of 20I?- Merhawi Plasmid Extraction/Microscopy transformation of expected vector- Merhawi? Latara? Chemical transformation- Khamani and MaryMade some primers for MCS of vector?- Khamani and Mary Electroporation- Khamani and Payal
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            <div class="date">15<span>jul</span></div>
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                <div class="extra-wrap">
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                <p><a href="#" class="link-1">week 8.</a></p>
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                    Payal, Marry, Khamani- Miniprep, restriction digest, gel electrophoresis of pGAP. Merhawi and Alyssa- Miniprep plus quantitative analysis, and culture colonies from plates
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            <div class="date">22<span>jul</span></div>
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                <p><a href="#" class="link-1">week 9.</a></p>
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                    Alyssa, Merhawi-Library research, wiki, plasmid digest. Khamani and Brandi- Vectors Payal & Mary- Plate to broth transfer of transformed pGap and Miniprep. Alyssa - midi prep of pORE vector
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            <div class="date">29<span>jul</span></div>
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                <p><a href="#" class="link-1">week 10.</a></p>
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                    Payal, Mahesh, Mary,- Electrocompetent cells, ran gels of midi and mini prep. Chlorophenicol research Latara- Research and planting
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            <div class="date">05<span>aug</span></div>
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                <div class="extra-wrap">
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                <p><a href="#" class="link-1">week 11.</a></p>
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                    Latara- Planting and Research Merhawi- Research
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            <div class="date">12<span>aug</span></div>
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                <p><a href="#" class="link-1">week 12.</a></p>
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                    Khamani- Primer Stock (resuspended dry oligos) Research for another binary vector - Dafna
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            <div class="date">19<span>aug</span></div>
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                <div class="extra-wrap">
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                <p><a href="#" class="link-1">week 13.</a></p>
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                    Merhawi- Watered plants Khamani- PCR of pGAP and restriction digest. Mary, Payal, Khamani, Krystle- Gel Electrophoresis
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            <div class="date">26<span>aug</span></div>
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                <div class="extra-wrap">
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                <p><a href="#" class="link-1">week 14.</a></p>
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                    Latara- Vector Design Khamani Robinson- Gel Extraction MCS and gel analysis Krystle- Ligation of linker
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            <div class="date">02<span>sept</span></div>
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                <div class="extra-wrap">
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                <p><a href="#" class="link-1">week 15.</a></p>
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                    Payal, Mary- LB broth, picked transformed cultures put into broth. Alyssa- Mini Prep Dafna - cell cultures of E4 vector
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Latara- Miniprep of 20I DNA and quantification and restriction digest Krystle- Miniprep of pGap with linker
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            <div class="date">09<span>sept</span></div>
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                <div class="extra-wrap">
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                <p><a href="#" class="link-1">week 16.</a></p>
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                    Merhawi, Kelsey, Alyssa Dafna - transformation of cell cultures from E4 vector and primer design for pPZP500 vector Latara- Miniprep and Restriction Digest of E4 vector Khamani- Club fair, wiki, cultures, research, miniprep Morgan- Miniprep with Khamani Alyssa - restriction digest and mini prep
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            <div class="date">16<span>sept</span></div>
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                <p><a href="#" class="link-1">week 17.</a></p>
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                    Khamani and Krystle- Restriction digest and pGap digest with linker gel Payal, Mary, Amish- wikim posters,broth Alyssa-Mini prep of LB broth + Ecoli cells with Austria Vector cultures, Restiction digest with EcoRI enzyme of mini prep samples and gel Electrophoresis. Gel showed no bands.- PCR of ecoli with Austria vector colonies (picked from LB/Kan plates), Gel electrophoresis of finished PCR samples, prepared LB/Spectinomycin agar Dafna:Poured LB/Spectinomycin plates Latara - transformation with Sarah and quanitfication
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            <div class="date">25<span>sept</span></div>
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                <div class="extra-wrap">
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                <p><a href="#" class="link-1">week 18.</a></p>
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                    Payal and Mary- PCR of mini preps and ran gel Dr. Brewer- Ran gel of PCR Krystle- PCR of mini preps and Midiprep of pGap alpha z with linker Khamani- YPD with zeocin medium plates and broth, ligation of RFP into pGap. Transformation into pichia Dafna - made LB agar plates with Spectinomycin and plate transformed pPZP500 vector Merhawi - transformation of pPZP500 vector Latara - PCR to get Kanamycin resistance out
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     </section>
     </section>

Revision as of 00:05, 4 October 2012

Safety

Safety

1. Would any of your project ideas raise safety issues in terms of researcher, public, or environmental safety?

The organisms used in our lab are well documented and generally regarded as safe (GRAS). These organisms are nonpathogenic and are not harmful for use in the lab. The GSU iGEM team follows lab safety very carefully. Lab goggles, long coats, gloves, and proper attire is always required while working in the lab. Additionally, all recombinant organisms were disposed of properly, ensuring no accidental release into the environment. Our project ideas did not raise any other safety issues. .

2. Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? If yes, did you document these issues in the Registry? How did you manage to handle the safety issue? How could other teams learn from your experience?

Activities in the development of BioBrick parts did not pose an issue to the GSU iGEM team. We created out own linker piece to fit the iGEM standard with no issues. This linker piece can be used to make most all commercially-available vectors fit the iGEM assembly standard. We fully comply with the National Institutes of Health Guidelines for Recombinant DNA.

3. Is there a local biosafety group, committee, or review board at your institution? If yes, what does your local biosafety group think about your project? If no, which specific biosafety rules or guidelines do you have to consider in your country?

GSU has an Institutional Biosafety Committee. The team has complied with all safety rules and regulations throughout the project. All routine lab inspections were conducted by the Biosafety Officer, and all members have been properly trained on autoclaving, dishwashing, and disposal of biohazardous and chemical wastes. The link for the GSU Biosafety Website is: http://www.gsu.edu/research/ibc.html

4. Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices, and systems be made even safer through biosafety engineering?

In the future, iGEM could provide teams with mini, online courses to give an overview/introduction of biosafety regulations and guidelines. The courses could be followed by a small quiz that all participating teams must pass. This would ensure that students have a better understanding of safety in the lab. In conjunction with GSU, all members working in a laboratory must pass the CITI online training.