"
Team:HokkaidoU Japan/Notebook
From 2012.igem.org
(Difference between revisions)
(→6th) |
|||
| Line 25: | Line 25: | ||
Transformation | Transformation | ||
*Transformation of BBa_B0015(dT), B0034(RBS), I179005(pT7), K346007(Ag43), and K542009(pLacI-RBS-Ag43)in DH5α | *Transformation of BBa_B0015(dT), B0034(RBS), I179005(pT7), K346007(Ag43), and K542009(pLacI-RBS-Ag43)in DH5α | ||
| - | ** | + | **Cultivated on LBA(dt,RBS,T7) and LBC(Ag43, pLacI-RBS-Ag43) |
| + | ***T7:21hrs Others:20hrs | ||
==5th== | ==5th== | ||
Transformation | Transformation | ||
*Transformation of K346007(Ag43)in DH5α. | *Transformation of K346007(Ag43)in DH5α. | ||
| - | ** | + | **Cultivated on LBC in 21hrs. |
Single colony isolation | Single colony isolation | ||
*Single colony isolation of BBa_B0015, B0034, I179005 and K542009. | *Single colony isolation of BBa_B0015, B0034, I179005 and K542009. | ||
| - | **BBa_K542009 was Ag43 only part! And the part didn't have Biobrick suffix. | + | **Cultivation on LBA(dt,RBS,T7) and LBC(pLacI-RBS-Ag43) in 14hrs30mins |
| + | ***BBa_K542009 was Ag43 only part! And the part didn't have Biobrick suffix. | ||
==6th== | ==6th== | ||
Liquid culture | Liquid culture | ||
*Liquid culture in LBA(dT,RBS,pT7) and LBC(pLacI-RBS-Ag43) | *Liquid culture in LBA(dT,RBS,pT7) and LBC(pLacI-RBS-Ag43) | ||
| - | **LB : 1ml is for glycerol stocks and 2ml is for mini-prep | + | **LB : 1ml is for glycerol stocks and 2ml is for mini-prep. |
Single colony isolation | Single colony isolation | ||
*Single colony isolation of K346007(Ag43). | *Single colony isolation of K346007(Ag43). | ||
Revision as of 12:06, 6 July 2012
| Home | Team | Official Team Profile | Project | Parts Submitted to the Registry | Modeling | Notebook | Safety | Attributions |
|---|
Contents |
Hello
We are team HokkaidoU Japan! Today we learn and start to edit wiki.
(>ω<)
Dear Mr.Ortiz, I saw the help page which you edited.
hola!
July
4th
Transformation
- Transformation of BBa_B0015(dT), B0034(RBS), I179005(pT7), K346007(Ag43), and K542009(pLacI-RBS-Ag43)in DH5α
- Cultivated on LBA(dt,RBS,T7) and LBC(Ag43, pLacI-RBS-Ag43)
- T7:21hrs Others:20hrs
- Cultivated on LBA(dt,RBS,T7) and LBC(Ag43, pLacI-RBS-Ag43)
5th
Transformation
- Transformation of K346007(Ag43)in DH5α.
- Cultivated on LBC in 21hrs.
Single colony isolation
- Single colony isolation of BBa_B0015, B0034, I179005 and K542009.
- Cultivation on LBA(dt,RBS,T7) and LBC(pLacI-RBS-Ag43) in 14hrs30mins
- BBa_K542009 was Ag43 only part! And the part didn't have Biobrick suffix.
- Cultivation on LBA(dt,RBS,T7) and LBC(pLacI-RBS-Ag43) in 14hrs30mins
6th
Liquid culture
- Liquid culture in LBA(dT,RBS,pT7) and LBC(pLacI-RBS-Ag43)
- LB : 1ml is for glycerol stocks and 2ml is for mini-prep.
Single colony isolation
- Single colony isolation of K346007(Ag43).
