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Team:HokkaidoU Japan/Notebook
From 2012.igem.org
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*Liquid culture in LBA(B0015,B0034) and LBC(K542009) | *Liquid culture in LBA(B0015,B0034) and LBC(K542009) | ||
**LB 3ul: 1ul is for glycerol stocks and 2ul is for mini-prep | **LB 3ul: 1ul is for glycerol stocks and 2ul is for mini-prep | ||
| + | |||
| + | Single colony isolation | ||
| + | *Single colony isolation of K346007(Ag43). | ||
Revision as of 11:24, 6 July 2012
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Contents |
Hello
We are team HokkaidoU Japan! Today we learn and start to edit wiki.
(>ω<)
Dear Mr.Ortiz, I saw the help page which you edited.
hola!
July
4th
Transformation
- Transformation of BBa_B0015(dT), B0034(RBS), I179005(pT7), K346007(Ag43), and K542009(pLacI-RBS-Ag43)in DH5α
- Cultivation on LBA(B0015,RBS) and LBC(Ag43, pLacI-RBS-Ag43)
5th
Transformation
- Transformation of K346007(Ag43)in DH5α.
- Cultivation on LBC.
Single colony isolation
- Single colony isolation of BBa_B0015, B0034, I179005 and K542009.
- BBa_K542009 was Ag43 only part! And the part didn't have Biobrick suffix.
6th
Liquid culture
- Liquid culture in LBA(B0015,B0034) and LBC(K542009)
- LB 3ul: 1ul is for glycerol stocks and 2ul is for mini-prep
Single colony isolation
- Single colony isolation of K346007(Ag43).
