Team:Washington/Protocols

From 2012.igem.org

(Difference between revisions)
 
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[https://2012.igem.org/Team:Washington/Protocols/osmY_Assay osmY Assay]
[https://2012.igem.org/Team:Washington/Protocols/osmY_Assay osmY Assay]
 +
[https://2012.igem.org/Team:Washington/Protocols/PUR_Assay PUR Esterase Assay]
[https://2012.igem.org/Team:Washington/Protocols/PUR_Assay PUR Esterase Assay]
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[https://2012.igem.org/Team:Washington/Protocols/EG_Assay Ethylene Glycol Assay]
[https://2012.igem.org/Team:Washington/Protocols/EG_Assay Ethylene Glycol Assay]

Latest revision as of 19:13, 3 October 2012


General Protocols

General Agarose Gel Electrophoresis

General SDS PAGE gel Electrophoresis

General PCR Protocol

General Digestion Protocol

General Ligation Protocol

General Transformation Protocol

Electroporation (Transformation)

Colony PCR Protocol

Competent Cell Prep Protocol

Standard 1L Expression Purification

Gene Assembly With Oligos

Sequencing

Computational Protein Design

Glycerol Stocks

Gibson Cloning/Assembly

Isolation of Plasmid DNA (miniprep)

Preparation of Overnight Cultures

PCR Purification

Gel Purification

Plastic Degradation

osmY Assay

PUR Esterase Assay

Ethylene Glycol Assay

Flu Binders

Kunkel Mutagenesis

[http://www.bio.davidson.edu/Courses/molbio/kunkel/kunkel.html Overview of the Kunkel Mutagenesis process]

Yeast Surface Display

Yeast Transformation

Optogenetics

Light App Experiments

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