"
Team:Columbia-Cooper-NYC/Plates
From 2012.igem.org
(Difference between revisions)
Ymakita (Talk | contribs)
(Created page with "= Protocol for Creating Plates = * Prepare 1L bottle * For 500mL, add 7.5g of agar and 12.5g of LB * Pour deionized water until water level reaches 500mL * Cover bottle with foil...")
Newer edit →
(Created page with "= Protocol for Creating Plates = * Prepare 1L bottle * For 500mL, add 7.5g of agar and 12.5g of LB * Pour deionized water until water level reaches 500mL * Cover bottle with foil...")
Newer edit →
Revision as of 01:59, 3 October 2012
Protocol for Creating Plates
- Prepare 1L bottle
- For 500mL, add 7.5g of agar and 12.5g of LB
- Pour deionized water until water level reaches 500mL
- Cover bottle with foil and tape with autoclave tape
- Autoclave on slow for 20 minutes
- After autoclaving, let bottle stand cool until touch
- Apply 500µl of 1000x stock antibiotics to make 1x solution
- Pour solution into plates
