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| - | <!-- *** What falls between these lines is the Alert Box! You can remove it from your pages once you have read and understood the alert *** -->
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| + | [[File:GeorgiaTech_tower.png|right|65px|link=http://www.gatech.edu]] |
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| - | <div id="template" style="text-align: center; font-weight: bold; font-size: large; color: #f6f6f6; padding: 5px;">
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| - | This is a template page. READ THESE INSTRUCTIONS.
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| - | <div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
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| - | You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples <a href="https://2009.igem.org/Help:Template/Examples">HERE</a>.
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| - | <div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #f6f6f6; padding: 5px;">
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| - | You <strong>MUST</strong> have all of the pages listed in the menu below with the names specified. PLEASE keep all of your pages within your teams namespace.
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| - | {|align="justify"
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| - | |You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.
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| - | |[[Image:Georgia_Tech_logo.png|200px|right|frame]]
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| - | ''Currently, scientific endeavors are being pursued to engineer a synthetic quorum sensing system in E. Coli to express green fluorescent protein (GFP) in response to exogenous quorum sensing molecules. However, certain limitation exists as regards to the time required for the expression of GFP fluorescence. Anywhere from thirty minutes to two hours is necessary from the addition of the autoinducer until sufficient GFP can be transcribed, translated, accumulated, and detected, which for some synthetic biology applications may be unacceptable.
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| - | To this end, the goal of our experiment is to develop an alternative method to quickly visualize the response of E. coli to exogenous autoinducer by expressing the already present fusion proteins of GFP to the autoinducer receptor. Specifically, the C-terminus end of the GFP protein would be fused to one copy of the receptor gene which will be TraR from Agrobacterium tumefaciens, with the N-terminus end fused to a second copy of the same receptor. The GFP/receptor domain will get continuously transcribed and translated in the bacteria but it will not be able to fluoresce as the domains do not constitute a functional GFP protein. This will be brought about by the dimerization of the TraR receptor in the presence of autoinducers. This alternative method of inducing GFP expression is hypothesized to be efficient and quicker than the methods currently used as it bypasses the transcription and translation machinery of the cell while expressing the GFP.
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| - | ''
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| - | |[[Image:Georgia_Tech_team.png|right|frame|Your team picture]]
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| - | |align="center"|[[Team:Georgia_Tech | Team Georgia_Tech]]
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| - | |}
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| - | <!--- The Mission, Experiments --->
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| - | {| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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| - | !align="center"|[[Team:Georgia_Tech|Home]]
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| - | !align="center"|[[Team:Georgia_Tech/Team|Team]]
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| - | !align="center"|[https://igem.org/Team.cgi?year=2012&team_name=Georgia_Tech Official Team Profile]
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| - | !align="center"|[[Team:Georgia_Tech/Project|Project]]
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| - | !align="center"|[[Team:Georgia_Tech/Parts|Parts Submitted to the Registry]]
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| - | !align="center"|[[Team:Georgia_Tech/Modeling|Modeling]]
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| - | !align="center"|[[Team:Georgia_Tech/Notebook|Notebook]]
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| - | !align="center"|[[Team:Georgia_Tech/Safety|Safety]]
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| - | !align="center"|[[Team:Georgia_Tech/Attributions|Attributions]]
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| - | |}
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