Team:Calgary/Notebook/Protocols/mgtacircuit

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{{Team:Calgary/TemplateNotebookOrange| TITLE=Characterization of mgtA circuit with S7 promoter| CONTENT=

  • Cells with the appropriate circuits were grown in LB overnight with 10mM MgCl2.
  • The next morning the cells were spun down and washed with M9 minimal media and appropriate amounts of MgCl2.
  • Cells were then aliquoted into 96 well plates in triplicates for each concentration and OD was measured at 600nm for every 4 hour for 8 hours.
  • Additionally, the cells were plated on the appropriate antibiotic in dilutions of 1:1000 and 1:10000 for measuring CFU.

    • Retrieved from "http://2012.igem.org/Team:Calgary/Notebook/Protocols/mgtacircuit"