Team:Evry/Notebook/July/2
From 2012.igem.org
(Difference between revisions)
Cyrpaut (Talk | contribs)
(Created page with "Today's achievements: 1) T10 bacteria transformation with DNA ligated on Friday (29/06)- protocol as before <br> 2) Transformed bacteria spreaded on Petri's plate- LB-agar-ampic...")
Newer edit →
(Created page with "Today's achievements: 1) T10 bacteria transformation with DNA ligated on Friday (29/06)- protocol as before <br> 2) Transformed bacteria spreaded on Petri's plate- LB-agar-ampic...")
Newer edit →
Revision as of 06:24, 4 July 2012
Today's achievements:
1) T10 bacteria transformation with DNA ligated on Friday (29/06)- protocol as before
2) Transformed bacteria spreaded on Petri's plate- LB-agar-ampiciline- incubation 37 degrees Celsius, overnight
3) SOC preparation:
1,4g Hanahan's Broth 0,18g Glucose 50mL MiliQ water
filtrated on microfilter (if you have more important volume, autoclave it)