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Team:Cornell/Notebook
From 2012.igem.org
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==Protocols== | ==Protocols== | ||
*[[Team:Cornell/Notebook/Miniprep|Miniprep]] | *[[Team:Cornell/Notebook/Miniprep|Miniprep]] | ||
| - | *[[Team:Cornell/Notebook/PCR|PCR]] | + | *[[Team:Cornell/Notebook/Vent_PCR|Vent PCR]] |
| + | *[[Team:Cornell/Notebook/Phusion_PCR|Phusion PCR]] | ||
*[[Team:Cornell/Notebook/Gel_electrophoresis|Gel Electrophoresis]] | *[[Team:Cornell/Notebook/Gel_electrophoresis|Gel Electrophoresis]] | ||
*[[Team:Cornell/Notebook/Gel_purification|Gel Purification]] | *[[Team:Cornell/Notebook/Gel_purification|Gel Purification]] | ||
Revision as of 21:54, 3 July 2012
| Home | Team | Project | Parts | Modeling | Notebook | Protocols | Safety | Attributions |
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Subprojects
- Arsenic reporter -- test a system for Shewanella detecting arsenates and activating the Mtr pathway to produce an electrical current
- nah operon -- an operon that converts napthalene into salicylate, allowing it to be detected by the salicylate reporter.
- Salicylate reporter -- a system for Shewanella detecting salicylate and activating the Mtr pathway to produce an electrical current
Protocols
- Miniprep
- Vent PCR
- Phusion PCR
- Gel Electrophoresis
- Gel Purification
- DNA Quantification
- Digestion Reaction
- Dephosphorylation
- PCR Clean Up
- Ligation Reaction
- Transformation of E. coli
- Transformation of Shewanella
- Sequencing
- Gibson Assembly
