Team:UIUC-Illinois/Project/Design

From 2012.igem.org

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<center><img src="https://static.igem.org/mediawiki/2012/e/e1/PUFPINwild.png" alt="PUFPINwild"></center><br/>
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<p>The blue symbol labeled PUF-PIN represents the gene that is expressed to produce a wild-type PUF fused to a PIN endonuclease. The comparison of this construct's results to the mutant PUF-PIN (labeled *PUF-PIN) our main source of experimental data.</p><br/><br/>
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<p>The blue symbol labeled PUF-PIN represents the gene that is expressed to produce a wild-type PUF fused to a PIN endonuclease. The comparison of this construct's results to the mutant PUF-PIN (labeled *PUF-PIN) our main source of experimental data.</p><br/>

Revision as of 03:45, 29 September 2012

Header

Project Design

Project Design

  • Overview
  • PUF+PIN Fusion
  • PUF+PIN Reporter
  • Non-Specific Control
  • Theoretical Results

    • Experimental Design


    In designing our project we based our quantitative tests on fluorescence measured by a plate fluorescence reader. Our constructs were created in ways that best suit providing evidence for our hypothesis of the PUF-PIN fusion protein showing endonuclease activity. Generally, our results were collected from quantifying two main PUF-PIN fusion protein types, a wild type and a mutant type, in different conditions. These two fusion proteins had recognition sites that differed by one base pair.

    Click on the list to the left to read about each of our constructs and why we decided to do them.

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