Team:Uppsala University/Parts

From 2012.igem.org

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<h3><b>Small RNA Scaffold</b></h3>
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<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K864440" style="margin-left:10px">BBa_K864440</a> - <b>J23101-Spot42</b>:
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<p>This is the native spot42 small RNA constitutively expressed by BBa_J23101 and is useful as a template for generating a randomized library of small RNAs for downregulation of any gene of interest.</p>
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<h3><b>Reporter System</b></h3>
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<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K864444" style="margin-left:10px">BBa_K864444</a> - <b>RFP-linker-SYFP</b>:
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<p>This part can be used to insert any 5’UTR of a gene of interest to screen for downregulation of this gene by artificial small RNAs. This reporter reporter system comes with a fluorescent marker that is downregulated by any small RNA targeting the 5'UTR of the gene of interest.</p>
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<h3><b>New low-copy backbone</b></h3>
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Screening system
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<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K864001" style="margin-left:10px">BBa_K864001</a> - <b>pSB4C15</b>:
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<p>This is a new BioBrick standard vector with low copy pSC101 replication origin, especially usable for Lambda Red recombineering in E coli.</p>
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aeBlue
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<h3><b>Blue Chromoprotein</b></h3>
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<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K864401" style="margin-left:10px">BBa_K864401</a> - <b>aeBlue</b>:
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pSB4C15
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<p>aeBlue is a blue chromoprotein extracted from the basal disk of a beadlet anemone Actinia equina. The protein has an absorption maximum at 597nm and a deep blue colour. The protein aeBlue has significant sequence homologies with proteins in the GFP family.</p>
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Latest revision as of 03:51, 27 September 2012

Team Uppsala University – iGEM 2012


Favorite Parts

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Small RNA Scaffold

BBa_K864440 - J23101-Spot42:

This is the native spot42 small RNA constitutively expressed by BBa_J23101 and is useful as a template for generating a randomized library of small RNAs for downregulation of any gene of interest.


Reporter System

BBa_K864444 - RFP-linker-SYFP:

This part can be used to insert any 5’UTR of a gene of interest to screen for downregulation of this gene by artificial small RNAs. This reporter reporter system comes with a fluorescent marker that is downregulated by any small RNA targeting the 5'UTR of the gene of interest.


New low-copy backbone

BBa_K864001 - pSB4C15:

This is a new BioBrick standard vector with low copy pSC101 replication origin, especially usable for Lambda Red recombineering in E coli.


Blue Chromoprotein

BBa_K864401 - aeBlue:

aeBlue is a blue chromoprotein extracted from the basal disk of a beadlet anemone Actinia equina. The protein has an absorption maximum at 597nm and a deep blue colour. The protein aeBlue has significant sequence homologies with proteins in the GFP family.

Submitted Parts

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BioBrick ID Type Name Description
BBa_K864100 Coding SYFP2 Yellow fluorescent protein
BBa_K864101 Composite B0032 - SYFP2 Yellow fluorescent protein
BBa_K864102 Composite B0034 - SYFP2 Yellow fluorescent protein
BBa_K864150 Composite cat-sacB Selection/counter-selection casette
BBa_K864200 Coding MarR Multi-resistant repressor
BBa_K864201 Coding LexA Repressor of the E. coli SOS-system
BBa_K864202 Coding MarR(G95S) Point-mutated for higher repression
BBa_K864203 Coding LexA(G85D) Point-mutated for higher repression
BBa_K864204 Coding OmpF Outer membrane porin
BBa_K864680 Coding TetM Ribosome protection protein
BBa_K592012 Coding eforRed Red/pink chromoprotein
BBa_K864401 Coding aeBlue Blue chromoprotein
BBa_K864402 Composite J23110 - B0034 - eforRed Red/pink chromoprotein
BBa_K864403 Composite J23110 - B0034 - aeBlue Blue chromoprotein
BBa_K864404 Composite J23110 - B0034 - cjBlue Turquoise chromoprotein
BBa_K864440 Scaffold J23101 - Spot42 Scaffold for sRNAs
BBa_K864444 Measurement RFP - linker - SYFP2 Screening system for sRNAs using a fluorescent marker
BBa_K592010 Coding amilGFP Yellow chromoprotein
BBa_K864600 Regulatory T0 Synthetic terminator
BBa_K864601 Regulatory TλT1 Synthetic terminator

Submitted backbones

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BioBrick ID Name Description
BBa_K864000 pSB4A15 Low copy BioBrick standard vector
BBa_K864001 pSB4C15 Low copy BioBrick standard vector
BBa_K864002 pSB4K15 Low copy BioBrick standard vector
BBa_K864003 pSB4S15 Low copy BioBrick standard vector
BBa_K864004 pSB4A15(FRT) Low copy BioBrick standard vector
BBa_K864005 pSB4C15(FRT) Low copy BioBrick standard vector
BBa_K864006 pSB4K15(FRT) Low copy BioBrick standard vector
BBa_K864007 pSB4S15(FRT) Low copy BioBrick standard vector
BBa_K864008 pSB4A15iq Low copy BioBrick standard vector with LacI
BBa_K864009 pSB4C15iq Low copy BioBrick standard vector with LacI
BBa_K864010 pSB4K15iq Low copy BioBrick standard vector with LacI
BBa_K864011 pSB4S15iq Low copy BioBrick standard vector with LacI
BBa_K864013 pSB4C15iq(FRT) Low copy BioBrick standard vector with LacI
BBa_K864014 pSB4K15iq(FRT) Low copy BioBrick standard vector with LacI
BBa_K864017 pSB8C15 Low copy BioBrick temperature sensitive standard vector
BBa_K864019 pSB8A15 Low copy BioBrick temperature sensitive standard vector
BBa_K864020 pSB8S15 Low copy BioBrick temperature sensitive standard vector



Sponsors






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