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Team:Tokyo Tech/Parts
From 2012.igem.org
(→Best improved part(s): BBa_K934012, BBa_K934024) |
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We constructed [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934022 BBa_K934022] by combining Plux promter ([http://partsregistry.org/wiki/index.php?title=Part:BBa_R0062 BBa_R0062]) and LasI ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K081016 BBa_K081016]). This is the first Biobrick part that succeeded in 3OC6HSL dependent 3OC12HSL production. Moreover, by co-culturing the cells containing this part with the cells containing 3OC6HSL dependent 3OC12HSL producer part ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934012 BBa_K934012]), we succeeded in constructing a positive feedback system. | We constructed [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934022 BBa_K934022] by combining Plux promter ([http://partsregistry.org/wiki/index.php?title=Part:BBa_R0062 BBa_R0062]) and LasI ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K081016 BBa_K081016]). This is the first Biobrick part that succeeded in 3OC6HSL dependent 3OC12HSL production. Moreover, by co-culturing the cells containing this part with the cells containing 3OC6HSL dependent 3OC12HSL producer part ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934012 BBa_K934012]), we succeeded in constructing a positive feedback system. | ||
| - | =Best improved part(s): [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934012 BBa_K934012], [http://partsregistry.org/wiki/index.php?title=Part: | + | =Best improved part(s): [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934012 BBa_K934012], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934025 BBa_K934025]= |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K934012 BBa_K934012]: | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934012 BBa_K934012]: | ||
By constructing [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934012 BBa_K934012], we improved a 3OC12HSL-dependent LuxI generator part. Even though a 3OC12HSL-dependent LuxI generator ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K266000 BBa_K266000]) was constructed by IPN-UNAM-Mexico 2009 team, no experimental data has been supplied. | By constructing [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934012 BBa_K934012], we improved a 3OC12HSL-dependent LuxI generator part. Even though a 3OC12HSL-dependent LuxI generator ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K266000 BBa_K266000]) was constructed by IPN-UNAM-Mexico 2009 team, no experimental data has been supplied. | ||
Moreover, [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0079 BBa_R0079], the lasI promoter used in [http://partsregistry.org/wiki/index.php?title=Part:BBa_K266000 BBa_K266000], was evaluated as an ineffective promoter in the Experience Page of the Registry. Thus, in this study, we constructed a new Plas-LuxI ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934012 BBa_K934012]) by using an effective lasI promoter ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K649000 BBa_K649000]) and confirmed its activity. This result can be considered as an improvement of the 3OC12HSL-dependent LuxI generator part. | Moreover, [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0079 BBa_R0079], the lasI promoter used in [http://partsregistry.org/wiki/index.php?title=Part:BBa_K266000 BBa_K266000], was evaluated as an ineffective promoter in the Experience Page of the Registry. Thus, in this study, we constructed a new Plas-LuxI ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934012 BBa_K934012]) by using an effective lasI promoter ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K649000 BBa_K649000]) and confirmed its activity. This result can be considered as an improvement of the 3OC12HSL-dependent LuxI generator part. | ||
| - | [http://partsregistry.org/wiki/index.php?title=Part: | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934025 BBa_K934025]: |
| - | By constructing [http://partsregistry.org/wiki/index.php?title=Part: | + | By constructing [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934025 BBa_K934025], we improved a Plux/tet hybrid promoter part. Even though a Plux/tet hybrid promoter was constructed by USTC 2009 team, their experiment did not contain the inducer combination: 3OC6HSL(-) and aTc(+). Thus, data of Plux/tet hybrid promoter in Biobrick Registry was not sufficient. In this study, we constructed a new Plux/tet hybrid promoter ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934025 BBa_K934025]) and confirmed its activity under all four combinations. This result can be considered as an improvement of the Plux/tet hybrid promoter part. |
</div> | </div> | ||
Revision as of 03:15, 27 September 2012
Parts
For a brief overview of our main results, please have a look at our Main Results page. [Go to "HOME"]
| Name | Type | Description | Designer | Length | |
| W | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934001 BBa_K934001] | Composite | phaC1-A-B1 [P(3HB) synthesis] | Taku Nakayama | 4208 |
| W | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934012 BBa_K934012] | Composite | Plas-LuxI | Toshiki Hashimoto | 755 |
| W | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934022 BBa_K934022] | Composite | Plux-LasI | Toshiki Hashimoto | 798 |
| Name | Type | Description | Designer | Length | |
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934016 BBa_K934016] | Composite | Plas-LacI | Naoki Tsukuda | 1332 | |
| W | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934024 BBa_K934024] | Regulatory | Plux/tet hybrid promoter | Toshiki Hashimoto | 72 |
| W | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934025 BBa_K934025] | Reporter | Plux/tet-GFP | Toshiki Hashimoto | 955 |
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934026 BBa_K934026] | Composite | Plux-LacI | Naoki Tsukuda | 1304 |
Best new BioBrick part (natural): [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934001 BBa_K934001]
We constructed a part containing phaC1-A-B1 in Biobrick format ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934001 BBa_K934001]). This is the first Biobrick part that succeeded in synthesizing Polyhydroxyalkanoates (PHAs).
Best new BioBrick part (engineered): [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934022 BBa_K934022]
We constructed [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934022 BBa_K934022] by combining Plux promter ([http://partsregistry.org/wiki/index.php?title=Part:BBa_R0062 BBa_R0062]) and LasI ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K081016 BBa_K081016]). This is the first Biobrick part that succeeded in 3OC6HSL dependent 3OC12HSL production. Moreover, by co-culturing the cells containing this part with the cells containing 3OC6HSL dependent 3OC12HSL producer part ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934012 BBa_K934012]), we succeeded in constructing a positive feedback system.
Best improved part(s): [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934012 BBa_K934012], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934025 BBa_K934025]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K934012 BBa_K934012]: By constructing [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934012 BBa_K934012], we improved a 3OC12HSL-dependent LuxI generator part. Even though a 3OC12HSL-dependent LuxI generator ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K266000 BBa_K266000]) was constructed by IPN-UNAM-Mexico 2009 team, no experimental data has been supplied. Moreover, [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0079 BBa_R0079], the lasI promoter used in [http://partsregistry.org/wiki/index.php?title=Part:BBa_K266000 BBa_K266000], was evaluated as an ineffective promoter in the Experience Page of the Registry. Thus, in this study, we constructed a new Plas-LuxI ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934012 BBa_K934012]) by using an effective lasI promoter ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K649000 BBa_K649000]) and confirmed its activity. This result can be considered as an improvement of the 3OC12HSL-dependent LuxI generator part.
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K934025 BBa_K934025]: By constructing [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934025 BBa_K934025], we improved a Plux/tet hybrid promoter part. Even though a Plux/tet hybrid promoter was constructed by USTC 2009 team, their experiment did not contain the inducer combination: 3OC6HSL(-) and aTc(+). Thus, data of Plux/tet hybrid promoter in Biobrick Registry was not sufficient. In this study, we constructed a new Plux/tet hybrid promoter ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934025 BBa_K934025]) and confirmed its activity under all four combinations. This result can be considered as an improvement of the Plux/tet hybrid promoter part.
<groupparts>iGEM012 Tokyo_Tech</groupparts>
