Team:Tokyo Tech/Parts

From 2012.igem.org

(Difference between revisions)
(Best improved part(s): BBa_K934012, BBa_K934024)
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We constructed [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934022 BBa_K934022] by combining Plux promter ([http://partsregistry.org/wiki/index.php?title=Part:BBa_R0062 BBa_R0062]) and LasI ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K081016 BBa_K081016]). This is the first Biobrick part that succeeded in 3OC6HSL dependent 3OC12HSL production. Moreover, by co-culturing the cells containing this part with the cells containing 3OC6HSL dependent 3OC12HSL producer part ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934012 BBa_K934012]), we succeeded in constructing a positive feedback system.
We constructed [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934022 BBa_K934022] by combining Plux promter ([http://partsregistry.org/wiki/index.php?title=Part:BBa_R0062 BBa_R0062]) and LasI ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K081016 BBa_K081016]). This is the first Biobrick part that succeeded in 3OC6HSL dependent 3OC12HSL production. Moreover, by co-culturing the cells containing this part with the cells containing 3OC6HSL dependent 3OC12HSL producer part ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934012 BBa_K934012]), we succeeded in constructing a positive feedback system.
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=Best improved part(s): [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934012 BBa_K934012], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934024 BBa_K934024]=
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=Best improved part(s): [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934012 BBa_K934012], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934025 BBa_K934025]=
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K934012 BBa_K934012]:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K934012 BBa_K934012]:
By constructing [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934012 BBa_K934012], we improved a 3OC12HSL-dependent LuxI generator part. Even though a 3OC12HSL-dependent LuxI generator ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K266000 BBa_K266000]) was constructed by IPN-UNAM-Mexico 2009 team, no experimental data has been supplied.  
By constructing [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934012 BBa_K934012], we improved a 3OC12HSL-dependent LuxI generator part. Even though a 3OC12HSL-dependent LuxI generator ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K266000 BBa_K266000]) was constructed by IPN-UNAM-Mexico 2009 team, no experimental data has been supplied.  
Moreover, [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0079 BBa_R0079], the lasI promoter used in [http://partsregistry.org/wiki/index.php?title=Part:BBa_K266000 BBa_K266000], was evaluated as an ineffective promoter in the Experience Page of the Registry. Thus, in this study, we constructed a new Plas-LuxI ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934012 BBa_K934012]) by using an effective lasI promoter ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K649000 BBa_K649000]) and confirmed its activity. This result can be considered as an improvement of the 3OC12HSL-dependent LuxI generator part.  
Moreover, [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0079 BBa_R0079], the lasI promoter used in [http://partsregistry.org/wiki/index.php?title=Part:BBa_K266000 BBa_K266000], was evaluated as an ineffective promoter in the Experience Page of the Registry. Thus, in this study, we constructed a new Plas-LuxI ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934012 BBa_K934012]) by using an effective lasI promoter ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K649000 BBa_K649000]) and confirmed its activity. This result can be considered as an improvement of the 3OC12HSL-dependent LuxI generator part.  
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K934024 BBa_K934024]:
+
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K934025 BBa_K934025]:
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By constructing [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934024 BBa_K934024], we improved a Plux/tet hybrid promoter part. Even though a Plux/tet hybrid promoter was constructed by USTC 2009 team, their experiment did not contain the inducer combination: 3OC6HSL(-) and aTc(+). Thus, data of Plux/tet hybrid promoter in Biobrick Registry was not sufficient. In this study, we constructed a new Plux/tet hybrid promoter ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934024 BBa_K934024]) and confirmed its activity under all four combinations. This result can be considered as an improvement of the Plux/tet hybrid promoter part.
+
By constructing [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934025 BBa_K934025], we improved a Plux/tet hybrid promoter part. Even though a Plux/tet hybrid promoter was constructed by USTC 2009 team, their experiment did not contain the inducer combination: 3OC6HSL(-) and aTc(+). Thus, data of Plux/tet hybrid promoter in Biobrick Registry was not sufficient. In this study, we constructed a new Plux/tet hybrid promoter ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934025 BBa_K934025]) and confirmed its activity under all four combinations. This result can be considered as an improvement of the Plux/tet hybrid promoter part.
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Revision as of 03:15, 27 September 2012

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PARTS

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Parts

For a brief overview of our main results, please have a look at our Main Results page. [Go to "HOME"]


Favorite Tokyo_Tech 2012 iGEM Team Parts


Name Type Description Designer Length
W[http://partsregistry.org/wiki/index.php?title=Part:BBa_K934001 BBa_K934001]CompositephaC1-A-B1 [P(3HB) synthesis]Taku Nakayama4208
W[http://partsregistry.org/wiki/index.php?title=Part:BBa_K934012 BBa_K934012]CompositePlas-LuxIToshiki Hashimoto755
W[http://partsregistry.org/wiki/index.php?title=Part:BBa_K934022 BBa_K934022]CompositePlux-LasIToshiki Hashimoto798
Tokyo_Tech 2012 iGEM Team Parts


Name Type Description Designer Length
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K934016 BBa_K934016]CompositePlas-LacINaoki Tsukuda1332
W[http://partsregistry.org/wiki/index.php?title=Part:BBa_K934024 BBa_K934024]RegulatoryPlux/tet hybrid promoterToshiki Hashimoto72
W[http://partsregistry.org/wiki/index.php?title=Part:BBa_K934025 BBa_K934025]ReporterPlux/tet-GFPToshiki Hashimoto955
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K934026 BBa_K934026]CompositePlux-LacINaoki Tsukuda1304

Best new BioBrick part (natural): [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934001 BBa_K934001]

We constructed a part containing phaC1-A-B1 in Biobrick format ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934001 BBa_K934001]). This is the first Biobrick part that succeeded in synthesizing Polyhydroxyalkanoates (PHAs).

Best new BioBrick part (engineered): [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934022 BBa_K934022]

We constructed [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934022 BBa_K934022] by combining Plux promter ([http://partsregistry.org/wiki/index.php?title=Part:BBa_R0062 BBa_R0062]) and LasI ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K081016 BBa_K081016]). This is the first Biobrick part that succeeded in 3OC6HSL dependent 3OC12HSL production. Moreover, by co-culturing the cells containing this part with the cells containing 3OC6HSL dependent 3OC12HSL producer part ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934012 BBa_K934012]), we succeeded in constructing a positive feedback system.

Best improved part(s): [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934012 BBa_K934012], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934025 BBa_K934025]

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K934012 BBa_K934012]: By constructing [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934012 BBa_K934012], we improved a 3OC12HSL-dependent LuxI generator part. Even though a 3OC12HSL-dependent LuxI generator ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K266000 BBa_K266000]) was constructed by IPN-UNAM-Mexico 2009 team, no experimental data has been supplied. Moreover, [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0079 BBa_R0079], the lasI promoter used in [http://partsregistry.org/wiki/index.php?title=Part:BBa_K266000 BBa_K266000], was evaluated as an ineffective promoter in the Experience Page of the Registry. Thus, in this study, we constructed a new Plas-LuxI ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934012 BBa_K934012]) by using an effective lasI promoter ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K649000 BBa_K649000]) and confirmed its activity. This result can be considered as an improvement of the 3OC12HSL-dependent LuxI generator part.

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K934025 BBa_K934025]: By constructing [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934025 BBa_K934025], we improved a Plux/tet hybrid promoter part. Even though a Plux/tet hybrid promoter was constructed by USTC 2009 team, their experiment did not contain the inducer combination: 3OC6HSL(-) and aTc(+). Thus, data of Plux/tet hybrid promoter in Biobrick Registry was not sufficient. In this study, we constructed a new Plux/tet hybrid promoter ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934025 BBa_K934025]) and confirmed its activity under all four combinations. This result can be considered as an improvement of the Plux/tet hybrid promoter part.

<groupparts>iGEM012 Tokyo_Tech</groupparts>