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Team:Uppsala University/Datapage
From 2012.igem.org
(Difference between revisions)
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<h3><b>Small RNA Scaffold</b></h3> | <h3><b>Small RNA Scaffold</b></h3> | ||
| - | <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K864440" style="margin-left:10px">BBa_K864440</a> - <b>J23101-Spot42</b>: | + | <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K864440" style="margin-left:10px">BBa_K864440</a> - <b>J23101-Spot42</b>: This is the native spot42 small RNA constitutively expressed by BBa_J23101 and is useful as a template for generating a randomized library of small RNAs for downregulation of any gene of interest. |
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<h3><b>Reporter System</b></h3> | <h3><b>Reporter System</b></h3> | ||
| - | <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K864444" style="margin-left:10px">BBa_K864444</a> - <b>RFP-linker-SYFP</b>: | + | <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K864444" style="margin-left:10px">BBa_K864444</a> - <b>RFP-linker-SYFP</b>: This part can be used to insert any 5’ÚTR of a gene of interest to screen for downregulation of this gene by artificial small RNAs. This reporter reporter system comes with a fluorescent marker that is downregulated by any small RNA targeting the 5'UTR of the gene of interest. |
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<h3><b>New low-copy backbone</b></h3> | <h3><b>New low-copy backbone</b></h3> | ||
| - | <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K864001" style="margin-left:10px">BBa_K864001</a> - <b>pSB4C15</b>: | + | <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K864001" style="margin-left:10px">BBa_K864001</a> - <b>pSB4C15</b>: This is a new BioBrick standard vector with low copy pSC101 replication origin, especially usable for Lambda Red recombineering in E coli. |
Revision as of 02:46, 27 September 2012
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Small RNA ScaffoldBBa_K864440 - J23101-Spot42: This is the native spot42 small RNA constitutively expressed by BBa_J23101 and is useful as a template for generating a randomized library of small RNAs for downregulation of any gene of interest.Reporter SystemBBa_K864444 - RFP-linker-SYFP: This part can be used to insert any 5’ÚTR of a gene of interest to screen for downregulation of this gene by artificial small RNAs. This reporter reporter system comes with a fluorescent marker that is downregulated by any small RNA targeting the 5'UTR of the gene of interest.New low-copy backboneBBa_K864001 - pSB4C15: This is a new BioBrick standard vector with low copy pSC101 replication origin, especially usable for Lambda Red recombineering in E coli.Blue ChromoproteinBBa_K864401 - aeBlue: | |||
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PromotortestBBa_K864401 - aeBlue:BackbonesBBa_K864401 - aeBlue:eforRedBBa_K864401 - aeBlue: | |||
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catSacBBBa_K864401 - aeBlue:IqBBBBa_K864401 - aeBlue:SP8BBBBa_K864401 - aeBlue: |
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