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Team:Caltech/Notebook/material/Biofuel Protocols
From 2012.igem.org
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<h3><a name="Ethanol_Assay"; style="color: #000000";>Ethanol Assay</a></h3> | <h3><a name="Ethanol_Assay"; style="color: #000000";>Ethanol Assay</a></h3> | ||
| + | <h4>Materials</h4> | ||
| + | <ul> | ||
| + | <li> 100 uL ethanol | ||
| + | <li> 5 mL water | ||
| + | </ul> | ||
| + | <h4>Method</h4> | ||
<p>The ethanol assay uses the following reagants and consists of the reaction depicted below. MTT Formazan appears purple, and it is due to this color change that the assay works.</p> | <p>The ethanol assay uses the following reagants and consists of the reaction depicted below. MTT Formazan appears purple, and it is due to this color change that the assay works.</p> | ||
| - | < | + | <ul> |
| + | <li>Make a serial dilution of ethanol. As shown below, we found a linear concentration to absorbance relation for concentrations under .05% ethanol. | ||
| + | |||
| + | </ul> | ||
| + | <h5>Ethanol Color Assay Reaction</h4> | ||
<img src="https://static.igem.org/mediawiki/2012/1/11/Ethanol_assay_reaction.png" alt="Photo of Reaction" title="Ethanol Color Assay" height="200px" width="700px" /> | <img src="https://static.igem.org/mediawiki/2012/1/11/Ethanol_assay_reaction.png" alt="Photo of Reaction" title="Ethanol Color Assay" height="200px" width="700px" /> | ||
| + | <br> | ||
Revision as of 22:38, 2 July 2012
Biofuel Protocols
Ethanol Assay
Materials
- 100 uL ethanol
- 5 mL water
Method
The ethanol assay uses the following reagants and consists of the reaction depicted below. MTT Formazan appears purple, and it is due to this color change that the assay works.
- Make a serial dilution of ethanol. As shown below, we found a linear concentration to absorbance relation for concentrations under .05% ethanol.
