File:GECO on plate reader 96 wells.jpg

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Latest revision as of 01:26, 27 September 2012

Samples of yeast on a 96-wells plate. Samples contain either the GECO-plasmid and CCH-MID1 channel plasmids (columns 5 to 10), only a GECO plasmid (column 3), only CCH1-MID1 plasmids (column 4), or no plasmids at all (column 2). Column 1 contains PBS. Furthermore, cells containing all three plasmids were cultured on medium with 2% glucose (column 5 to 7) or on medium with 2% galactose (column 8 to 10). Since the GECO protein is under control of the GAL-promotor, only in galacose medium the GECO protein is expressed. Vertically, cells are put into either PBS (row A), PBS+0.5% Tween (row B), PBS+0.1 mM calcium acetate (row C) and PBS+0.5% Tween+0.1 mM calcium acetate (rowD). Rows E to H are filled with supernatant of induced cells to determine the background fluorescence of the medium.

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	Date/Time	Thumbnail	Dimensions	User	Comment
current	22:35, 26 September 2012		733×514 (68 KB)	Nickveeken (Talk \| contribs)

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Camera manufacturer	Samsung
Camera model	Galaxy Nexus
Exposure time	9,999/250,000 sec (0.039996)
F Number	f/2.75
Date and time of data generation	18:04, 14 September 2012
Lens focal length	3.43 mm
Horizontal resolution	72 dpi
Vertical resolution	72 dpi
Software used	Paint.NET v3.5.10
File change date and time	11:49, 19 September 2012
Y and C positioning	1
Exposure Program	Aperture priority
Exif version	2.2
Date and time of digitizing	18:04, 14 September 2012
Image compression mode	0
Shutter speed	4.525
Aperture	2.75
Brightness	0
Exposure bias	0
Maximum land aperture	2.75
Subject distance	0 meters
Metering mode	MultiSpot
Light source	Fluorescent
Flash	Flash did not fire
Color space	sRGB
Flash energy	0
Sensing method	One-chip color area sensor
Custom image processing	Custom process
Exposure mode	Auto exposure
White balance	Auto white balance
Digital zoom ratio	1
Scene capture type	Standard
Contrast	Normal
Saturation	Normal
Sharpness	Normal
Subject distance range	Unknown
GPS date	14 September 2012

Revision as of 22:35, 26 September 2012 (view source) Nickveeken (Talk \| contribs) ← Older edit	Latest revision as of 01:26, 27 September 2012 (view source) Nickveeken (Talk \| contribs)
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-	+	Samples of yeast on a 96-wells plate. Samples contain either the GECO-plasmid and CCH-MID1 channel plasmids (columns 5 to 10), only a GECO plasmid (column 3), only CCH1-MID1 plasmids (column 4), or no plasmids at all (column 2). Column 1 contains PBS. Furthermore, cells containing all three plasmids were cultured on medium with 2% glucose (column 5 to 7) or on medium with 2% galactose (column 8 to 10). Since the GECO protein is under control of the GAL-promotor, only in galacose medium the GECO protein is expressed. Vertically, cells are put into either PBS (row A), PBS+0.5% Tween (row B), PBS+0.1 mM calcium acetate (row C) and PBS+0.5% Tween+0.1 mM calcium acetate (rowD). Rows E to H are filled with supernatant of induced cells to determine the background fluorescence of the medium.