"
Team:Evry-Genopole/Notebook/July/2
From 2012.igem.org
(Difference between revisions)
(Created page with "Today's achievements: 1) T10 bacteria transformation with DNA ligated on Friday (29/06)- protocol as before 2) Transformed bacteria spreaded on Petri's plate- incubation 37 degr...") |
|||
| Line 1: | Line 1: | ||
Today's achievements: | Today's achievements: | ||
| - | 1) T10 bacteria transformation with DNA ligated on Friday (29/06)- protocol as before | + | 1) T10 bacteria transformation with DNA ligated on Friday (29/06)- protocol as before <br> |
| - | 2) Transformed bacteria spreaded on Petri's plate- incubation 37 degrees Celsius, overnight | + | 2) Transformed bacteria spreaded on Petri's plate- incubation 37 degrees Celsius, overnight <br> |
| - | 3) SOC preparation: | + | 3) SOC preparation: \n |
1,4g Hanahan's Broth | 1,4g Hanahan's Broth | ||
0,18g Glucose | 0,18g Glucose | ||
50mL MiliQ water | 50mL MiliQ water | ||
filtrated on microfilter (if you have more important volume, autoclave it) | filtrated on microfilter (if you have more important volume, autoclave it) | ||
Revision as of 15:56, 2 July 2012
Today's achievements:
1) T10 bacteria transformation with DNA ligated on Friday (29/06)- protocol as before
2) Transformed bacteria spreaded on Petri's plate- incubation 37 degrees Celsius, overnight
3) SOC preparation: \n
1,4g Hanahan's Broth
0,18g Glucose
50mL MiliQ water
filtrated on microfilter (if you have more important volume, autoclave it)
