Diary July

8-14

Meeting :
lux team member: Jinhee LEE, Sumiya KAWAI, Miho NARITA, Tomomi YOKOYAMA, Mayumi NAKAMURA
rhodopsin team member: Hiroto OKANO, Genki SAKAI, Hayato TSURUTA, Somin LEE

August
5-11

Meeting :
Small session on gene experiments using iGEM biobrick.

Lux team :
Design primers to clone lux operon from photobacterim phosphorium genome DNA.

19-25

Rhodopsin team :
construction of blue light sensor.
Pconst.(H)-RBS-NpSRII-9a.a.linker_NpHtrII-EnvZ-DT
sequence analysis of constructed blue light sensor biobrick.

Meeting :
lux team and rhodopsin team made small presentation about experiments.

26-9/1

Rhodopsin team :
construction of ⊿EnvZ competent cell

Meeting :
Our project title and team character was desided!
“Coli express for long distance communication”

Lux team :
lux operon was cloned from photobacterim phosphorium genome DNA. Most sequence was confirmed by sequence analysis.

2-8

Meeting :
lux team and rhodopsin team made small presentation about experiments.

Rhodopsin team :
insertion of PompR(+)-RBS-GFP-DT under blue light sensor biobrick.(BBa~)
plasmid extraction of blue light sensor + GFP and sequence analysis.

9-15

Lux team :
lux bio-brick (pSB1C3-PBAD-RBS-lux operon-DT) was constructed! E. coli TOP10 was transformed with this plasmid.
Evaluation of lux bio-brick. Luminescence was measured using plate reader. We could see luminescence in dark room.
Design primers to clone rib operon from E.coli genome DNA
lux bio-brick that constitutive expression of lux gene was constructed. We expected more luminescence using strong constitutive expression promoter.

Rhodopsin team :
evaluation of blue light sensor.
After preculture, transformants were culture under blue light or dark condition.
But cells were not growth well.

Meeting :
Our team T-shirt design idea has been completed.


16-22

Lux team :
rib operon was cloned fromE.coli genome DNA. Most sequence was confirmed by sequence analysis
Evaluation of color change biobrick.

Meeting :

Presentation team
Poster session team
We began to design poster and slide.


23-29

Rhodopsin team :
Reevaluation of blue light sensor.
After preculture, transformants were culture under white light or dark condition.

Lux team :
evaluation of fast luminescence biobrick.

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