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Team:Paris-Saclay/Project/Notebook/Week 7
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Latest revision as of 00:30, 27 September 2012
Week 7
- PCR in large volume to increase the amount of available biobricks.
- Treatment with DPN1 to eliminate plasmids.
- Gel purification to only keep fragments of interest.
- Gibson Assembly method to put together the different biobricks for each construction.
- Transformation with assembly products and staggering on a selective medium.
- Colony PCR to have results before the transformation results.
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