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Team:Paris-Saclay/Project/Notebook/Week 4
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Latest revision as of 00:29, 27 September 2012
Week 4
- We placed an order for the primers.
- DNA (biobricks) Resuspension using iGEM protocol.
- Thermal shock treatment of each biobrick to have a large quantity of DNA.
- Staggering of the transformed bacteria on a selective medium to select bacteria which recieved the plasmid.
- We started some colonies, one per biobrick, on a liquid culture, in order to make a Miniprep.
- Miniprep to purify each biobrick (ready to use).
- Groove for two colonies because they did not grow on the liquid culture.
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