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Team:Paris-Saclay/Project/Notebook/Week 5
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[[Category:Team:Paris-Saclay/Project Gemote/Notebook|e]] | [[Category:Team:Paris-Saclay/Project Gemote/Notebook|e]] | ||
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| - | *End of liquid culture (for the grooving colonies) | + | *End of liquid culture (for the grooving colonies). |
| - | *End of Miniprep | + | *End of Miniprep : we have usable biobricks. |
| - | *Culture stocks for each biobrick, to have a reserve of DNA | + | *Culture stocks for each biobrick, to have a reserve of DNA. |
| - | *PCR to amplify the different biobricks we will need to put together the different fragments using Gibson Assembly Method | + | *PCR to amplify the different biobricks we will need to put together the different fragments using the Gibson Assembly Method. |
| - | *Electrophoresis gel to verify the length of each | + | *Electrophoresis gel to verify the length of each biobrick. |
| - | *Purification of the PCR products | + | *Purification of the PCR products when we had the correct length. |
| - | + | ||
{{Team:Paris-Saclay/Follow}} | {{Team:Paris-Saclay/Follow}} | ||
Revision as of 00:25, 27 September 2012
Week 5
- End of liquid culture (for the grooving colonies).
- End of Miniprep : we have usable biobricks.
- Culture stocks for each biobrick, to have a reserve of DNA.
- PCR to amplify the different biobricks we will need to put together the different fragments using the Gibson Assembly Method.
- Electrophoresis gel to verify the length of each biobrick.
- Purification of the PCR products when we had the correct length.
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