From 2012.igem.org

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Revision as of 00:14, 27 September 2012

Notebook

May

1st week: 5/27-6/2

May 29th

Making LB medium

May 30th

Making LB Agar
Making LB Amp Plates

May 31st

Preculture

June

2nd week: 6/3-6/9

June 5th

Plating JM109

June 6th

preculture

June 7th

Measuring OD by spectroscope
Meking electrocompetent cells
Electroporating of a test plasmid

June 8th

Caluculatiing transformation efficiency

3rd week: 6/10-6/16

4th week: 6/17-6/23

5th week: 6/24-6/30

June 26th

Making LB tetracycline plate

June 27th

June 28th

Making LB kanamycin plate and chloramphenicol plate
Transformation with a test plasmid

June 29th

Transformation with #1(constitutive promoter) and #11(RBS)

2012/07

6th week: 7/1-7/7

July 3rd

Making K solution
Colony PCR DNA fragment assumed as fhlA (#6) for cloning

July 4th

Miniprep #1, #11

July 5th

Making 1% agarose solution
Electrophoresis (#6)
Digest (#1_EP, #11-_EP)

July 6th

Colony PCR (#6)

7th week: 7/8-7/14

July 9th

Electrophoresis (#6)

July 10th

Transformation
- #3(RBS medium), #5(d.term), #20(pLac)

July 11th

Preculture
- #3, #5, #20
Gel Extraction(#6)

July 12th

The continuation of Gel Extraction(#6)
Miniprep
- #3, #5, #20

July 13th

Nanodrop (#6).
Digestion (#3:E-P, #5:E-P, #20:E-P)
Electrophoresis (#3, #5, #20)

July 14th

Miniprep (#1,3,5,11,20)
Gel extraction (#6)

8th week: 7/15-7/21

July 17th

Transformation (#1A2,#3,#5,#20)
PCR (parts of 1. and #3,#20(Colony PCR))
Incubate #5.

July 18th

Miniprep (#1A2,#3,#5,#20)
PCR using u.p (#1A2,#3,#5,#20)
Colony PCR (#3,#20,H₂O)
Electrophoresis (#1A2,#3,#5,#20, #3,#20, H₂O)
PCR (2011’s #1,#36 and 2012’s #3,#5,#20, H₂O)

July 20th

Miniprep.
Electrophoresis (7/19’s PCR product(#1,#3,#5,#20,#36) )
Colony PCR (#3,#5,#20)

July 21st

Electrophoresis (7/20’s PCR product (#3,#5,#20))

9th week: 7/22-7/28

July 24th

Making agar medium plate contain ampicillin
PCR (#6, #8)
Electrophoresis (#6, #8)
Transformation (#1)
Culture (#5, #20)

July 25th

Miniprep (#5, #20)
Purify (#6, #8)
Digestion (#3:S-P,#5:E-X,#11:E-S,#20:S-P)
Electrophoresis (#5, #11)
Ligation (#5,#11) (?)

July 26th

Culture (#1)
Digestion (#3:S-P, #5:E-X, #11:E-S, #20:S-P, fhlA:E-S, hycA:E-S)
Electrophoresis (#3:S-P, #5:E-X, #11:E-S, #20:S-P, fhlA:E-S, hycA:E-S)
Gel extraction (#3:S-P, #5:E-X, #11:E-S, #20:S-P, fhlA:E-S, hycA:E-S)

July 27th

Culture, Miniprep (#1)
Digestion (#5-A:E-X, #11-A:X-P)
Electrophoresis (#3:S-P, #20:S-P, #11:E-S)
Gel extraction (#3:S-P, #20:S-P, #11:E-S, E-S vector, hycAp:E-S)

July 28th

Digestion(#1,3,5,11,hycAp)
Gel extraction(#1,3,5,11,hycAp)
Cloning(fhlA)

10th week: 7/29-8/4

July 30th

Digestion (#3:S-P, #11:X-P)
Electrophoresis (#3, #11)
Gel extraction (#3, #11)
Ligation (#3, #11)
Transformation (ligation product; #3-11)

August 1st

Digestion (#3:S-P, #11:X-P)
Electrophoresis (#3, #11)
Gel extraction (#3, #11)
Ligation (#3, #11)
Transformation (ligation product; #3-11)
Miniprep (#3, #5, #11)

August 2nd

Colony PCR (#3-11)
Culture (#3-11)
Colony pick up, culture (red colony of #11)
Digestion (#3:S-P, #5:E-X)

August 3th

Gel extraction (#3:S-P, #5:E-X)
Making solution of Kanamycin (100mg/mL)
Culture, miniprep (#11)
Culture (#11)

August 4th

Plating, Miniprep (#11)

11th week: 8/5-8/11

August 6th

Culture, Colony PCR (#3-11)

August 7th

Colony PCR, culture (#3-11)
Miniprep, Digestion, Gel extraction (culture product; #3-11)

August 8th

Digestion (#5: E-X)
Gel extraction (#5)
Miniprep (#5)
Colony PCR (culture product; #3-11)

August 9th

Colony PCR (#3-11)
Culture, Miniprep (#11)
Digestion (#11:X-P)
Cloning (#6,#8)
Ligation
Gel extraction (ligation product; #5, #11)
Ligation, Transformation (#3, #11)

August 10th

Colony PCR (#3-11)
Miniprep (#3)
Digestion (#3:S-P)
Cloning, Colony PCR (#6,#8)
cut check, PCR check (#3-11)
Electrophoresis (ligation product; #3, #11, PCR product; #6)
Gel extraction (#6)

August 11th

electrophoresis(PCR product; #3-11)
Digestion (#6:E-S)
electrophoresis (#8)

12th week: 8/12-8/18

August 13th

Check, Culture (#3-11)
Transformation (test plasmid)

August 15th

Gel extraction (cut check product; #3, #11)
Ligation, Transformation (#3-#11)
Make Vector for #6
Digestion (#3: E-S)
Gel extraction (digestion product; #3, #6(8/11)
PCR check (ligation product; #3-11)
Cloning (#6)

August 16th

Gel extraction (#6)
cloning (#6)
PCR check (#3-11)
PCR-cleanup (#8)
culture (#3-11)

August 17th

electrophoresis (#6)
Miniprep (#3-11)
Digestion (#3-#11: E-S, #6: E-S)
Gel extraction (#3-11)
Ligation, Transformation (#3-11-5)
Making solution of Ampicillin

13th week: 8/19-8/25

August 20th

PCR check (#3-11-5)
Digestion (#8: E-S)
Cloning (#6)
Digeston (#8: E-S)
Gel extraction (#6)

August 21th

Colony PCR (#1)
Digestion (#6: E-S, #3-11: E-S, #20: S-P)
Ligation (#3-11, #5)
Gel extraction (#3-11, #6)
Ligation (#6-pSB1A2)
Electrophresis (#3, #5; made previously)

August 22th

Cloning (#6; from test plasmid)
Colony PCR (#3-11-5(colony is not red), #20, 8/21 ligation product;#6, #8)
Miniprep,Frozen stock (culture product; #1, #5, #20)
Transformation (#8, #3-11-5)
Digestion (#6:X-S)
Making solution of Ampicillin
cloning retry (#6)

August 23th

PCR check (ligation product; #6)
Cloning (#6)

Colony PCR (#1, #6, #8, #11, #3-11-5, #3-11)

August 24th

Digestion (#6, E-S, #3-11, E-S)
Colony PCR #3-11
Electrophresis, Gel extraction (#6)
Vector insert (#6)
Sequencing (#8)

August 25th

Digestion (#6: E-S)
Colony PCR (#6-Vector)
Gel extraction (#3-11)

14th week: 8/26-9/2

August 26th

Electrophresis (#6)
Master plate (#6)
Colony PCR (#3-11-5)
Preculture (#6)

August 27th

Ligation (Miniprep product; #3-11, #5)
Digestion retry (#3-11, #5)
Miniprep (#6)
Mutagenesis (#6)
Sequencing (#6)

Follow @UT_Tokyo

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-This is a template page. READ THESE INSTRUCTIONS.
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-<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
-You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
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-You <strong>MUST</strong>  have all of the pages listed in the menu below with the names specified.  PLEASE keep all of your pages within your teams namespace.
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-<!-- *** End of the alert box *** -->
-{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
-!align="center"|[[Team:UT-Tokyo|Home]]
-!align="center"|[[Team:UT-Tokyo/Team|Team]]
-!align="center"|[https://igem.org/Team.cgi?year=2012&team_name=UT-Tokyo Official Team Profile]
-!align="center"|[[Team:UT-Tokyo/Project|Project]]
-!align="center"|[[Team:UT-Tokyo/Parts|Parts Submitted to the Registry]]
-!align="center"|[[Team:UT-Tokyo/Modeling|Modeling]]
-!align="center"|[[Team:UT-Tokyo/Notebook|Notebook]]
-!align="center"|[[Team:UT-Tokyo/Safety|Safety]]
-!align="center"|[[Team:UT-Tokyo/Attributions|Attributions]]
-|}
-You should make use of the calendar feature on the wiki and start a lab notebook.  This may be looked at by the judges to see how your work progressed throughout the summer.  It is a very useful organizational tool as well.
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